Download Spin Cartridge Nucleic Acid Isolation Kit

Spin Cartridge Nucleic Acid Isolation Kit
Manual spin kit
Spin cartridge method realizes higher purity and higher yield in nucleic acid isolation.
Washing and recovery of nucleic acid can be performed in table-top compact centrifuges!
Enables rapid
isolation compared
to other methods
Rapid
Safe
Requires no hazardous
solvents such as
phenol / chloroform
High
Purity
High
yield
Further eliminates
impurities such as
chaotropic salts
Stably offers high yield
User -friendly wide-necked cartridge
QuickGene
Porous membrane
Conventional Glass
Fiber membrane
Revolutionary membrane realizes high purity and high yield
The nucleic acid isolation kit uses patented porous membrane. It is only 80μm thick,
thinner than the glass fiber membrane. Because of the outstanding adsorptive and
desorptive performances of the membrane, nucleic acid can be reliably isolated.
50µm
50µm
All-in-one package
‧Electron micrographs of cross sections of the membranes
Reagents, enzymes and vessels required for nucleic acid isolation are included in a
single package.
Work efficiency can be improved without purchasing new equipment
DNA/ RNA can be rapidly and easily isolated using equipment already available in
your laboratory, such as centrifuges and microtubes. Because washing and recovery
of nucleic acid can be performed in table-top compact centrifuge, work efficiency can
be drastically improved.
80um
1000um
QuickGene
glass fiber membrane
12.5times thinner
Easier, safer and more versatile nucleic acid isolation accelerates your research.
The wide-necked cartridge
enables easy application of
samples. It improves workability
and prevents loss of valuable
sample isolation.
Wide-necked cartridge
structure for easy
liquid application
The kit requires no hazardous
organic solvents such as phenol /
chloroform. The DNase-free and
RNase-free solvents used in
isolation processes are also
environmentally friendly
Harmless nucleic acid
isolation to researchers
and environment
Six isolation kits support various samples
Appropriate isolation kit selectable depending on sample (human/ mouse/ plants/ fungi etc.).
DNA/RNA can be rapidly and easily isolated from various organisms and parts.
DNA
RNA
1.DNA whole blood kit (spin method)
4.RNA tissue kit (spin method)
QuickGene SP kit DNA whole blood
Isolation example
Human, cow,
poultry etc.
QuickGene SP kit RNA tissue
Human, cow,
poultry,
mouse,
insects etc.
ca. 5ug / Whole blood 200ul
1. Pretreatment enzyme
3. Wash buffer
5. Cartridges
2. Lysis buffer
4. Elution buffer
6. Waste tubes
Isolation example
1. Lysis buffer
3. Wash buffer
5. Cartridges
2.DNA tissue kit (spin method)
Isolation example
QuickGene SP kit RNA cultured cell
Cultured cells
(floating/
adhesive),
plants etc.
ca. 4ug / 5mg Balb/c Mouse tail
1.Pretreatment enzyme 2. Tissue lysis buffer
3. Lysis buffer 4. Wash buffer 5. Elution buffer
6. Cartridges
7. Waste tubes
Isolation example
1.Lysis buffer
4. Cartridges
3. Plasmid kit (spin method)
2. Wash buffer
5. Waste tubes
3. Elution buffer
QuickGene SP kit RNA cultured cell HC
ca. 2.5ug / 1ml LB medium
6cm/ 10cm
dish cultured
cells
E. coli
1. Pretreatment enzyme
3. Resuspension buffer
5. Neutralization buffer
8. Cartridges
ca. 10ug / 1×106 cell HL60 cell
6.RNA cultured cell HC kit (spin method)
QuickGene SP kit plasmid
Isolation example
2. Solubilization buffer
4. Elution buffer
6. Waste tubes
5.RNA cultured cell kit (spin method)
QuickGene SP kit DNA tissue
Human, mouse,
fish and shellfish,
plants, cell lines,
fungi etc.
ca. 137ug / 30mg Mouse liver
2. Lysis buffer
4. Alkaline solution
6. Wash buffer 7. Elution buffer
9. Waste tubes
Isolation example
ca. 213ug / 10cm dish cultured HEK 293 cell
1. Lysis buffer
3. Wash buffer
5. Cartridges
2. Solubilization buffer
4. Elution buffer
6. Waste tubes
Comparison of workflow of DNA Isolation from human whole blood
The kit requires shorter time for Isolation compared to other companies' kits.
1min
1min
1min
1min
1min
1min
1min
Centrifuge
14,000rpm
1min
Stand Still
Centrifuge
8,000rpm
Centrifuge
Centrifuge
Incubate
8,000rpm
3min
8,000rpm
*Can be performed in table-top centrifuges.
Research Use Only
KURABO INDUSTRIES LTD.
Bio-Medical Department
14-5, Shimokida-cho, Neyagawa-shi, Osaka 572-0823, Japan
Phone: +81-72-820-3079
FAX: +81-72-820-3095
Email: [email protected]
www.kurabo.co.jp/bio/English
Genomic DNA
Centrifuge
8,000rpm
Elution buffer
Centrifuge
8,000rpm
Wash buffer
Centrifuge
8,000rpm
Wash buffer
Centrifuge
Voltex 15sec
Special-grade ethanol
10min
Apply whole lysate to cartridge
Incubate
Voltex 15sec
Lysis buffer
Whole blood 200μl
Pretreatment enzyme
Company A’s spin column
1.5ml microtube
QuickGene SP kit
2min
8,000rpm