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EXPRESSION OF WT1 AND CLAUDIN-5 IN ANGIOGENIC TUMORS
S. Gobbo, A. Eccher, F. Comper, M. Brunelli, D. Segala, I. Cataldo,
F. Menestrina, A. Scarpa, G. Martignoni
1Anatomia
Patologica - Università di Verona
Background
Identification of endothelial cells is based on the expression of
angioblastic linage-specific genes like CD31, CD34.
WT1:
protein is a zinc finger transcription factor
expressed in endothelial progenitor cells that has
been reported to be maintained during the
differentiation.
Claudin-5:
key component of tight endothelial
junctions.
Methods
Cases:
6
5
4
angiomas
Kaposi’s sarcomas
angiosarcomas
Immunohistochemistry:
WT1:
anti-WT1, clone 6F-H2 from Dako
diluted 1:100
Claudin-5:
anti-claudin-5, clone 4C3C2 from Zymed
diluted 1:100
ANGIOMA
WT1
Claudin-5
KAPOSI SARCOMA
WT1
Claudin-5
ANGIOSARCOMA
WT1
Claudin-5
WT1 EXPRESSION
ANGIOMA
KAPOSI
ANGIOSARCOMA
Results:
ANGIOMA
WT1:
claudin-5:
consistent cytoplasmic and membranous
immunoexpression
expressed expecially on the luminal membrane
with a week cytoplasmic labeling.
KAPOSI
WT1:
claudin-5:
strong cytoplasmic and membranous expression
strong cytoplasmic and membranous expression
ANGIOSARCOMA
WT1:
claudin-5:
strong immunoexpression in only 2 of 4 (50%)
expression in the cytoplasm and on the membrane
Conclusions:
WT1 and claudin-5 are specific endothelial markers
and are manteined in angiomas and Kaposi’s
sarcomas.
Angiosarcomas mantein the expression of claudin-5
whereas WT1, that has been proven to behave as a
tumor supressor protein, tends to be lost in the
development of the neoplasm.