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I. Antibodies:
i) Unc18-2( I-14) SC-23014, anti-goat, polyclonal (For transfected constructs)
ii) Unc18-1 (P-17) SC-14557, anti-goat, polyclonal (rat brain lysate)
iii) Septin7 (H-120) SC-20620, anti-rabbit, polyclonal
iv) ChromograninA (C-20), SC-1488, anti-goat, polyclonal
v) Synapsin2, SIGMA: 077K4877, anti-rabbit, polyclonal
vi) Synaptotagmin 5, BD: 612284, anti-mouse, monoclonal
vii) Myc, DSHB 910E, anti-mouse, monoclonal
viii) JH-93, (N-terminal ACTH), anti-rabbit, polyclonal (Secretory granule marker)
ix) HA, Thermo-scientific, PA1985, anti-rabbit, polyclonal ( substitute for HA, CAB3872, anti-rabbit)
x) GAM-CY3, Invitrogen A10521
XI) GAR-FITC, SC2012, Santa Cruz
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