Download Supplemental Table 9

Supplemental Data Table 9. Calculation of the amount of fetal DNA in the urine produced during each studied time interval.
Fractional
Amount of
fetal DNA
Fetal DNA
concentration
in urine
(%)
(GE)e
180,140
8.5
15,300
23.4
830,439
24.7
204,768
45,936
13.3
345,779
15.7
54,185
2,826
152,607
12.5
1,219,570
7.9
96,340
278
7,265
2,015,921
15.9
12,714,894
2.7
348,566
6 h - 12 h post-delivery
300
5,924
1,777,344
61.5
2,889,403
< LOD
−
12 h - 18 h post-delivery
710
1,478
1,049,025
63.3
1,656,963
< LOD
−
Pre-delivery
58
335
19,411
66.0
29,425
1.9
550
0 - 40 min post-delivery
488
75
36,551
90.8
40,257
4.3
1,729
40 - 70 min post-delivery
290
40
11,525
89.7
12,850
4.0
517
70 min - 2 h post-delivery
485
139
67,221
97.2
69,151
1.8
1,265
2 h - 6 h post-delivery
1,380
168
231,674
96.9
239,051
< LOD
−
6 h - 12 h post-delivery
600
2,371
1,422,564
40.0
3,561,164
< LOD
−
12 h - 18 h post-delivery
1,670
117
194,689
80.5
241,957
< LOD
−
Pre-delivery
47
6,037
283,742
27.1
1,047,389
17.6
184,472
0 - 40 min post-delivery
350
782
273,693
13.6
2,006,165
21.3
428,073
40 - 70 min post-delivery
365
135
49,151
85.6
57,415
< LOD
−
70 min - 2 h post-delivery
193
341
65,747
27.0
243,522
2.8
6,845
2 h - 6 h post-delivery
295
4,287
1,264,665
22.5
5,616,839
< LOD
−
6 h - 12 h post-delivery
188
7,920
1,489,009
39.7
3,748,056
< LOD
−
12 h - 18 h post-delivery
157
8,784
1,379,038
39.2
3,516,913
< LOD
−
Total urine
Leptin DNA
Total
Proportion
Corrected
volume
concentration
urine DNA
of urine DNA
total urine
(mL)a
(GE/mL)
(GE)b
≥ 62bp (%)c
DNA (GE)d
Pre-delivery
109
686
74,746
41.5
0 - 40 min post-delivery
113
1,728
194,362
40 - 70 min post-delivery
24
1,914
70 min - 2 h post-delivery
54
2 h - 6 h post-delivery
Urine collection
time period
Case 6
Case 7
Case 8
a
Total urine volume (mL), all the urine collected in the catheter bag during the studied time interval.
b
Total urine DNA (GE) = Total urine volume (mL) × Leptin DNA concentration (GE/mL).
c
Proportion of urine DNA ≥ 62 bp (%), proportion of urine DNA with size larger than or equal to 62bp (amplicon size of the leptin assay) which is
determined from the paired-end sequencing data.
Total urine DNA (GE)
Proportion of urine DNA ≥62 bp (%)
d
Corrected total urine DNA (GE) =
e
Amount of fetal DNA in urine (GE) = Corrected total urine DNA (GE) × Fractional fetal DNA concentration (%)