Download Table S1. Primers Gene primer sequence annealing temperature(°C

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Table S1. Primers
Gene
primer sequence
GPER
GAPDH
annealing temperature(°C)
FORWARD: GGGGAAGAGGCCACCAACAT
59
REVERSE: CTCACTCTCTGGGTACCTGGGTT
59
FORWARD: AACGGATTTGGTCGTATTG
59
REVERSE: GGAAGATGGTGATGGGATT
59
GPER
FORWARD: GGTTTCGTGAGCTACCACCA
60
Promoter 1
REVERSE: ACACGTCTACATTGGGCAGG
60
GPER
FORWARD: CAGAGGGTCACGGCGAATTA
60
Promoter 2
REVERSE: AGTTCATGCAGAGTGGGACG
60
Figure legend
Cell cycle profiles of ISHIKAWA and HEC-1A cells treated by insulin (100nM) and/or E2 (10nM)
for 48h. Insulin promoted estrogen-driven cell cycle progression by decreasing the level of
G0/G1 phase and increasing the level of S phase significantly. Insulin and estrogen
co-treatment increased the level of G2/M phase but not significantly when compared to E2
alone.
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