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Human KvLQT1/minK Channel Stable Cell Line
Host cell:
Human embryonic kidney (HEK)
Gene name:
KCNQ1/KCNE1
Growth media:
DMEM, 10% FBS
Storage:
Frozen under liquid nitrogen
Functional characteristics: Mean current amplitude ± SE = 1.7 ± 0.1nA (n=152)
Assay Platform:
Manual or automated patch clamp
Note:
KvLQT1/minK comprise the pore forming and auxiliary subunits
of the slow component (IKs) of the delayed rectifier potassium
current (IK).
Data:
B
current amplitude (pA)
A
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cell #
Figure: A. Recording of KvLQT1/mink current elicited by a ventricular action potential waveform in
the absence and presence of quinine. B. Current amplitude plot taken from 152 cells. All recordings
were performed at 37oC using manual patch clamp.
See: Crumb WJ Jr, Vicente J, Johannesen L, Strauss DG. An evaluation of 30 clinical drugs against the comprehensive in vitro proarrhythmia
assay (CiPA) proposed ion channel panel. J Pharmacol Toxicol Methods. 2016 Apr 6. pii: S1056-8719(16)30017.
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