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Download NEB Competent E. coli Strain Selection | NEB
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Home › Tools & Resources › NEB Competent E. coli Strain Selection NEB Competent E. coli Strain Selection Product NEB # Characteristics Strains for Cloning and Subcloning NEB® Stable Competent E. coli (High Efficiency) C3040H C3040I High quality plasmid preparations due to endA mutation Rapid growth recA strain T1 phage resistance (fhuA) Value pricing Free of animal products NEB® Turbo Competent E. coli (High Efficiency) C2984H C2984I Fastest growth – colonies visible after 6.5 hours Plasmid preparation after 4 hours Tight control/expression of toxic genes (F´lacI8) NEB® 5-alpha Competent E. coli (High Efficiency) C2987H C2987I C2987P C2987R C2987U Versatile cloning strain DH5α™ derivative NEB® 5-alpha F' Iq Competent E. coli (High Efficiency) C2992H C2992I Toxic gene cloning F´ strain with extremely high transformation efficiency NEB® 10-beta Competent E. coli (High Efficiency) C3019H C3019I Large plasmid and BAC cloning DH10B™ derivative dam-/dcm- Competent E. coli C2925H C2925I Dam/Dcm methyltransferase free plasmid growth NEB® Express Competent E. coli (High Efficiency) C2523H C2523I Versatile non-T7 expression strain Protease deficient NEB® Express Iq Competent E. coli (High Efficiency) C3037I Control of IPTG induced expression from Plac, Ptac,Ptrc and T5lac Strains for Protein Expression Protease deficient T7 Express Competent E. coli (High Efficiency) C2566H C2566I Most popular T7 expression strain Protease deficient T7 Express lysY Competent E. coli (High Efficiency) C3010I T7 expression Protease deficient Better reduction of basal expression T7 Express lysY/Iq Competent E. coli (High Efficiency) C3013I T7 expression Protease deficient Highest level of expression control T7 Express Crystal Competent E. coli (High Efficiency) C3022I T7 expression Protease deficient SeMet labeling for protein crystallography SHuffle® Express Competent E. coli C3028J Protease deficient/B strain Enhanced capacity to correctly fold proteins with multiple disulfide bonds in the cytoplasm SHuffle® T7 Express Competent E. coli C3029J T7 expression Protease deficient/B strain Enhanced capacity to correctly fold proteins with multiple disulfide bonds in the cytoplasm Product NEB # Characteristics SHuffle® T7 Express lysY Competent E. coli C3030J T7 expression Protease deficient/B strain Tightly controlled expression of toxic proteins Enhanced capacity to correctly fold proteins with multiple disulfide bonds in the cytoplasm SHuffle® T7 Competent E. coli C3026J T7 expression/K12 strain Enhanced capacity to correctly fold proteins with multiple disulfide bonds in the cytoplasm BL21 Competent E. coli C2530H Routine non-T7 expression BL21(DE3) Competent E. coli C2527H C2527I Routine T7 expression Lemo21(DE3) Competent E. coli C2528J Tunable T7 expression for difficult targets NiCo21(DE3) Competent E. coli C2529H Improved purity of target proteins isolated by IMAC There are many properties to consider when choosing a strain for your experiments. Requirements such as plasmid preparation, blue-white screening, cytoplasmic disulfide bond formation and fast colony growth necessitate specific strain choices. Please check the Strain Properties Chart for a particular experiment. DH5α™ and DH10β™ are trademarks of Invitrogen. SHuffle® is a registered trademark of New England Biolabs, Inc.
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