Download Kocaeli Üniversitesi

XIVth PRACTICAL COURSE ON BASIC STEM CELL
LABORATORY TECHNIQUES AND MOLECULAR BIOLOGY
APPLICATIONS
MARCH 21-25, 2011
KOU-KOGEM
CHAIRMAN:
PROF.DR. ERDAL KARAÖZ
SCIENTIFIC ORGANIZATION COMMITTEE
PROF.DR. ERTAN URAL
ASSOC.PROF.DR. ERAY ÇALIŞKAN
ASSOC.PROF.DR MURAT KASAP
ASSIST.PROF.DR AYLA EKER SARIBOYACI
ASSIST.PROF.DR. GÜLÇİN GACAR
ASSIST.PROF.DR. HALİME KENAR
ASSIST.PROF.DR GÜRALP ÖZKOÇ
DR. PINAR ÇETİNALP DEMİRCAN
DR. GÖKHAN DURUKSU
DR. GÜRLER AKPINAR
MSc. AYÇA AKSOY
MSc. Z. SEDA ÜNAL
MSc. ÖZLEM SAĞLAM
MSc. GÜLAY ERMAN
Bio. CANSU SUBAŞI
Lab. ALPASLAN OKÇU
SPONSORS:
NUMBER OF PARTICIPANTS:
Limited to 18 (participants will be separated into 2 groups)
LOCATION:
KOU-CENTER FOR STEM CELL AND GENE THERAPIES
RESEARCH AND PRACTICE, SCHOOL OF MEDICINE,
UMUTTEPE CAMPUS, IZMIT/KOCAELI / TURKEY
KOU-KÖK HÜCRE VE GEN TEDAVİLERİ ARAŞTIRMA VE
UYGULAMA MERKEZİ, TIP FAK. MORFOLOJİ BİNASI,
UMUTTEPE YERLEŞKESİ, İZMİT/KOCAELİ
ACCOMMODATION:
PASHA PALAS HOTEL (4 STAR) (TEL. +90-262 311 00 11)
COURSE FEE:
Academic members, Specialist
Assistant, Biologist, Student
Representative of firms
: 1500 TL
: 1250 TL
: 1500 TL
Services included in the registartion fees; Course program and book, name tag, certificate, symposium bag,
gala dinner, tea-coffee services and lunches during the Course
PREREQUISITE:
All life science researchers and students can participate our course
CERTIFICATE:
Successful participants are issued with a certificate by Kocaeli
University KOGEM and Continuing Education Centre (bilingual/
Eng.-Tur.). An additional “Flow-Cytometry” training certificate will
be issued by BD compony.
ADMISSION:
Please contact by e-mail ([email protected])
CONTACT:
Prof.Dr. Erdal Karaöz
Kocaeli Üniversitesi, Kök Hücre ve Gen Tedavileri Araştırma ve
Uygulama Merkezi.
Tıp Fakültesi Morfoloji Binası, Kat.1.
Umuttepe Yerleşkesi, Üçtepeler/İzmit/Kocaeli
Tel: +90-262-3038685
[email protected]
http://kogem.kocaeli.edu.tr
COURSE PROGRAM
DAY 1- 21.03.11 (dd.mm.yy) MONDAY
08.30-09.00
09.00-09.30
09.30-10.20
10.30-11.20
11.30-12.20
12.30-13.00
: Registration
: Introductory Lecture
: Stem Cell Biology and Mesenchymal
Stem Cells (MSCs)
: Technical Properties of the Stem Cell Culture
Lab.and Aseptic Conditions
: Basic Stem Cell Laboratory Techniques:
primary, suspension, monolayer and 3D cultures
: Isolation techniques of human and rodent MSCs
from peripheral/cord blood/bone marrow/
compact bone- negative/positive selection;
EasySep, RosetteSep, RoboSep, StemSep.
Prof.Dr. E. Karaöz
Prof.Dr. E. Karaöz
Assist. Prof. Dr. H. Kenar
Assist.Prof.Dr. A.E. Sarıboyacı
Bio. T. Akyüz (Amphi)
13.00-14.00: Lunch Break
14.00-18.00
: HANDS ON APPLICATION (KÖGEM/STEM CELL LABS)
Isolation and culture of MSCs from rat bone marrow
Isolation and culture of MSCs from human adipose tissue
Isolation and culture of MSCs from human peripheral/
umbilical cord blood
(E.KARAÖZ, A.E.SARIBOYACI, H.KENAR, A.AKSOY, A.OKÇU)
Isolation techniques of human and rodent MSCs from peripheral/cord
blood/bone marrow/compact bone- negative/positive selection; EasySep,
RosetteSep, RoboSep, StemSep. (E.KARAÖZ, T. AKYÜZ, A.AKSOY,
A.OKÇU)
DAY 2- 22.03.11 (dd.mm.yy) TUESDAY
09.00-09.50
10.00-10.50
11.00-11.30
11.30-12.00
12.00-12.30
: Subculturing, Cell Counting Techniques
and Cryopreservation techniques
: Characterization of MSCs and Evaluation of
Apoptosis by Flow Cytometry
: Cell cycle by Flow Cytometry
: Cellular senescence
: ELISA applications in stem cell studies
MSc.A.Okçu
Assist.Prof.Dr.G.Gacar
Bio. G.Erman
Assist. Prof. Dr. H. Kenar
MSc. Z.S. Genç
12.30-13.00: Lunch Break
13.00-15.00
: HANDS ON APPLICATION (KÖGEM/CELL CULTURE LABS)
Sub-culture, cell counting techniques, cryopreservation (freeze-thaw)
techniques
(E.KARAÖZ, A.E.SARIBOYACI, H.KENAR, A.OKÇU, A.AKSOY)
15.00-16.00
: APPLICATION (KÖGEM/ FLOW-CYTOMETRY LAB.)
Flow cytometry practical applications
(immunophenotypic analysis of MSCs from human
adipose tissue and apoptosis detection through Annexin V)
(G.GACAR, P.Ç.DEMİRCAN, G.BAYAZIT)
16.00-17.00
: APPLICATION (KÖGEM/STEM CELL LAB II)
ELISA/ Insulin detection
(Z.S. ÜNAL)
: APPLICATION (KÖGEM/ IMMUNOHISTOCHEMISTRY
17.00-18.00
LAB.)
Cell senesence test (beta-Gal staining)
(H.KENAR, C.SUBAŞI)
DAY 3- 23.03.11 (dd.mm.yy) WEDNESDAY
09.00-09.50
10.00-10.30
10.40-11.20
11.30-12.00
: Western Blotting
Assoc.Prof.Dr. M. Kasap
: Cell Proliferation Index and Viability Tests (MTT,XTT,WST1) Dr.P.Ç. Demircan
: Stem Cells and Proteomics
Dr. G. Akpınar
: Telomeraze Enzyme Activity and Telomere Length
Dr. G. Duruksu
12.00-13.00: Lunch Break
13.00-14.00
: APPLICATION (KOGEM/MOLECULAR BIOLOGY LAB I and II)
14.00-17.00
Aplication and Assesment of MTT and WST Tests
(P.Ç.DEMİRCAN, A.AKSOY, A.OKÇU)
: APPLICATION (KOGEM/MOLECULAR BIOLOGY LAB I and II)
Protein isolation
SDS-PAGE and Western blotting
(M.KASAP, G.GACAR, Z.S.GENÇ, Ö.SAĞLAM,G.DURUKSU)
Introduction of proteomix lab.
(M.KASAP, G.AKPINAR)
DAY 4- 24.03.11 (dd.mm.yy) THURSDAY
09.00-09.50
10.00-10.30
10.40-11.30
11.30-12.00
: RNA Isolation, cDNA Synthesis and PCR
: Gel Electrophoresis, Imaging Systems and Analyzing
: Gene Transfer Techniques
: Apoptosis and TUNEL Staining Technique
Assist.Prof.Dr. G. Gacar
MSc. Ö. Sağlam
Dr.G. Duruksu
MSc. B.Tekin (Medsantek)
12.00-13.00: Lunch Break
13.00-16.00
16.00-17.00
16.00-17.00
16.00-17.00
: APPLICATION (KÖGEM/ MOLECULAR BIOLOGY LAB I and II)
RNA isolation and cDNA synthesis, PCR and analysis of PCR
products
Agarose gel elektrophoretic analyses of RNA and DNA
(M.KASAP, G.GACAR, Z.S. GENÇ, Ö.SAĞLAM,G.DURUKSU)
: APPLICATION (KÖGEM/ STEM CELL LAB II)
Gene transfer applications (Transformation of GFP
gene into MSCs and screening under fluoresence microscope)
(G.DURUKSU)
: APPLICATION (KÖGEM/IMMUNHISTOCHEMICAL LAB.)
Apoptosis and TUNEL staining technique
( C.SUBAŞI, B.TEKİN)
: UYGULAMA (KÖGEM/ STEM CELL LAB I)
Microscopical examination of Stem Cells in primer culture condition
( E.KARAÖZ, A.AKSOY,A.OKÇU)
GALA DINNER
DAY 5- 25.03.11 (dd.mm.yy) FRIDAY
09.00-09.50
10.00-10.50
: In-vitro Differentiation Techniques
: Overview of Tissue Engineering Applications
MSc. A. Aksoy
Assist.Prof.Dr.G. Özkoç
11.00-11.50
12.00-12.30
: Mesenchymal Stem Cells and Tissue Engineering
: Immunohistochemical techniques in cell culture
Assist.Prof.Dr. H. Kenar
Bio. C.Subaşı
12.00-13.00: Lunch Break
13.00-15.00
: APPLICATION (KÖGEM/CELL CULTURE LABS)
Application of in-vitro differentiation techniques
(E.KARAÖZ, A.E.SARIBOYACI, A.AKSOY, A.OKÇU)
Seeding of MSCs on scaffolds in tissue engineering studies
(E.KARAÖZ, A.E.SARIBOYACI, H. KENAR, A.AKSOY, A.OKÇU)
15.30-16.30
: CERTIFICATE CEREMONY