Download Rad54: Rad51 Interaction

-The DNA translocation activity (forming positive and negative
supercoils) is required for all other Rad54 activities in the cell
Mazin et al. 2010
-The chromatin remodeling and protein (histone) displacement activities
free the DNA for processing by other proteins (such as Rad51 and Rad52)
Mazin et al. 2010
-Rad54 stabilizes the Rad51 nucleoprotein filament and also stimulates
stand invasion
Mazin et al. 2010
-Rad54 can both stabilize the Rad51 filament (in the presence of Ca2+)
and dissociate Rad51 (in the absence of Ca2+)
-Dissociation of Rad51 is a result of the translocation activity
Mazin et al. 2010
-The branch migration activity allows for processing of HR intermediates
-Mus81 activation by Rad54 resolves HR intermediates
Mazin et al. 2010
-All Rad54 activities (with the exception of Rad51 stabilization)
require the ATPase activity
Walker A Box
[G,X,G,K,S/T]
-Structure of the “Walker A Box”
-Site of ATP binding and hydrolysis
T184
Zebrafish Rad54
C-terminal domain
K183
L181
Thoma et al. 2005
-PCNA Interacting Protein sequence Box (PIP Box) (Burgess et al)
OR
-DNA binding sequence extending Motif III (Zhang et al)
-Both agree this sequence is necessary for ATPase activity
-Tyrosine and Phenylalanine mutants have
shown significant decreases in activity
Thoma et al. 2005
-No significant hydrophobic residues with some more significant
hydrophilic residues
-Consistent with a DNA-interacting protein