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Chromatin Immunoprecipitation Assay (ChIP)
1. Crosslink Protein-DNA complexes in situ
2. Isolate nuclei
and fragment DNA (sonication or digestion)
3. Immunoprecipitate with antibody 4a. Identify
against target nuclear protein
and reverse crosslinks
protein
components of
isolated complexes
4b. Identify
DNA
sequence by PCR,
cloning and
sequencing
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