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Protein Characterization Quotation Request Form Please complete the form and submit to [email protected]. Our service representative will contact you shortly with a quote. Page 1 of 3 Customer Information If you have an existing account with ProteinCT, just fill in your name and email address or your Account No. Name of Requestor: Account No.: Name of PI: First: Last: Middle: Title: Organization: Phone: Fax: Email Address: Shipping Address Project Information Nature of Inquiry Pricing estimation Quote for ordering Estimated Project Initiation Immediately >6 months 1-3 months 3-6 months Other, please specify Research Use Only In vitro diagnostics (IVD) Intended Use For grant application purpose Antibody drug development Protein Characterization Services Please fill out details for each selected services on the following page Category Protein Identification Services: Protein Modification and Conformation Analysis Services: Services Sample Requirement Protein MW determination service Purified protein samples Protein ID service Purified protein samples or SDS PAGE gel slices N-terminal sequencing Protein blot onto PVDF membrane Protein posttranslational modification (PTM) analysis service Purified protein samples or SDS PAGE gel slices Protein conformation analysis service Purified protein samples Protein metal co-factor analysis service Purified protein samples Protein Characterization Quotation Request Form Please complete the form and submit to [email protected]. Our service representative will contact you shortly with a quote. Page 2 of 3 Service Specifications – Protein Identification Services Please fill in project requirement details. Protein MW determination service: (if submitting more than 1 sample, please duplicate this table and fill out required information) Sample name: Target: Protein amount: Protein concentration: Target Mass range to be detected: Protein name: Accession number: Species: Protein sequence: MW: µg Requirement: Highly purified protein sample, 50µg or more; please include a buffer control Protein concentration: µg; sample volume: ml; Buffer: Da Comments: Protein ID service: (if more than 1 sample, please duplicate this table and fill out required information) Sample name: Target: Protein name: Accession number: Species: MW: Protein sequence: Protein amount: Purified sample: Requirement: Purified protein sample, 5µg or more; please include a buffer control Protein amount µg Protein concentration: µg; sample volume: ml; Buffer: Gel Slice: Protein amount Gel description: Gel %; µg (at least 1 µg per gel slice) Gel type Target Mass range to Da be detected: Comments: N-terminal sequencing service: (if more than 1 sample, please duplicate this table and fill out required information) Sample name: Target: Protein name: Accession number: Species: MW: Protein sequence: Protein amount: Target Mass range to be detected: Comments: Purified sample: Requirement: Purified protein sample, 50µg or more protein amount µg; Protein concentration: µg; sample volume: PVDF blot: Protein amount Da µg (5-10 µg per blot spot) ml Protein Characterization Quotation Request Form Please complete the form and submit to [email protected]. Our service representative will contact you shortly with a quote. Page 3 of 3 Service Specifications – Protein Modification and Conformation Analysis Services Please fill in project requirement details. Protein posttranslational modification (PTM) analysis: (if submitting more than 1 sample, please duplicate this table and fill out required information) Sample name: Target: Protein name: Accession number: Protein amount: PTM of interest: Target molecular weight: Species: MW: Protein sequence: Possible modification site description: Purified sample: Requirement: Purified protein sample, 50µg or more; please include a buffer control Protein amount µg Protein concentration: µg; sample volume: ml; Buffer: Gel Slice: Protein amount µg (at least 5-10 µg per gel slice) Gel description: Gel %; Gel type phosphorylation methylation glycosylation: please specify type Other: please specify Da Comments: Protein conformation analysis service: (if more than 1 sample, please duplicate this table and fill out required information) Sample name: Target: Protein amount: Protein name: Accession number: Species: Protein sequence: MW: Requirement: Purified protein sample, ≥ 0.5mg/ml, least 1ml or more; please include a buffer control Purified sample: Protein amount mg Protein concentration: mg; Sample volume: ml; Control buffer: volume: ml; Comments: Protein metal co-factor analysis service: (if more than 1 sample, please duplicate this table and fill out required information) Sample name: Target: Protein amount: Metals to be detected: Comments: Protein name: Accession number: Species: MW: Protein sequence: Please indicate mutations/variants as compared to the sequence corresponding to the accession number. Requirement: Purified protein sample, 100µg or more , with buffer control protein amount µg; Protein concentration: µg; sample volume: ml Control buffer: volume: ml; Circle metals to be detected: Zinc, Manganese, Copper, Iron, Sodium, Aluminum, Cadmium, Chromium, Cobalt, Lithium, Molybdenum, Nickel, Lead, Titanium, Vanadium and Selenium.