Download Supplemental Figure 2

Supplemental Figure 2. Mapping of miR161-and TAS2 3’D6(-)-mediated cleavage sites in PPR
gene transcripts by 5’ RACE. (A) Five PPR gene diagrams. Thick black lines represent open
reading frames (ORFs), flanking thin lines represent untranslated regions (if known), and carrots
represent introns. The putative miRNA and tasiRNA interaction sites are shown as gray boxes.
Horizontal arrows labeled with Roman numerals indicate the relative size of products using genespecific 5’ RACE primers. (B) Ethidium bromide-stained agarose gel showing the 5’ RACE
products for three PPR genes targeted by TAS2 3’D6(-) and miR161. Products from both wild-type
and hen-1 cDNA are shown. Roman numerals below each band correspond to 5’ RACE products
indicated in panel (A). Lane M, 100 base-pair ladder. Lane sets 1-3, At1g62910 5’ RACE products.
Lane sets 4-5, At1g63150 5’ RACE products. Lane sets 6-7, At1g63080 5’ RACE products. (C)
miR161- and TAS2 3’D6(-)-guided cleavage sites in five PPR transcripts. Roman numerals indicate
target sites in (A) and bands on agarose gel in (B). Bases in bold indicate predicted cleavage sites.
The fraction of cloned 5’ RACE PCR products terminating at a given position is indicated above
each duplex. For three of the diagrams, miR161.1 and miR161.2 target sites are indicated by italics
and underlines, respectively.