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Human Immunodeficiency Virus-1–Infected Macrophages
Induce Inducible Nitric Oxide Synthase and Nitric Oxide
(NO) Production in Astrocytes: Astrocytic NO as a Possible
Mediator of Neural Damage in Acquired Immunodeficiency
Syndrome
by Kotaro Hori, Parris R. Burd, Keizo Furuke, Joseph Kutza, Karis A. Weih, and
Kathleen A. Clouse
Blood
Volume 93(6):1843-1850
March 15, 1999
©1999 by American Society of Hematology
NO is produced and iNOS is expressed in cocultures of HIV-1–infected MDM and human
astrocytes.
Kotaro Hori et al. Blood 1999;93:1843-1850
©1999 by American Society of Hematology
Cells producing iNOS in cocultures are astrocytes but not MDM. MDM precultured for 7 days in
DMEM medium were infected with HIV-1ADA.
Kotaro Hori et al. Blood 1999;93:1843-1850
©1999 by American Society of Hematology
Coculture supernatant induces iNOS in astrocytes.
Kotaro Hori et al. Blood 1999;93:1843-1850
©1999 by American Society of Hematology
iNOS is induced by gp41 in cocultures of uninfected MDM and astrocytes.
Kotaro Hori et al. Blood 1999;93:1843-1850
©1999 by American Society of Hematology
NO inhibits HIV-1 replication in MDM. HIV-1ADA was incubated with or without SNAP for 3 hours
and then MDM was precultured for 7 days in DMEM medium were infected with non–SNAPtreated and SNAP-treated viruses.
Kotaro Hori et al. Blood 1999;93:1843-1850
©1999 by American Society of Hematology
A NOS inhibitor partially reverses the HIV-1 suppressive effect of astrocytes.
Kotaro Hori et al. Blood 1999;93:1843-1850
©1999 by American Society of Hematology
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