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Untreated Bleomycin g-H2AX DAPI Supplementary Figure S2: DSB-induced nuclear staining for g-H2AX. Ax2 cells were left untreated or exposed to 100mU/ml of bleomycin for 1 hour. Cells were processed, stained with antibodies raised against g-H2AX, and subjected to immunofluorescence as described in Materials and Methods. Several representative patterns of DSB-induced g-H2AX staining are illustrated.