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Table S2. List of 33 deletion mutants that are resistant in at least 3 of the 4 independent repeats of the screen. Their CLS phenotypes and S. cerevisiae orthologs are indicated. Systematic name SPCC188.02 SPAC1399.04c SPAC22E12.14c SPAC1687.15 SPAC17C9.02c SPAC3A12.09c SPAC806.07 SPBC16E9.13 SPBC1D7.01 SPBP4H10.16c SPCC1902.01 SPAC17G6.08 SPAC521.03 SPBC3H7.03c SPAC21E11.04 SPAC1556.02c SPCC16A11.08 SPAC16E8.01 SPAC17H9.08 SPAC20H4.10 SPAC3H1.08c SPBC21C3.08c SPBC31F10.03 SPAC323.03c SPAC3F10.04 SPAC821.07c SPBC1198.11c SPBC16D10.08c SPBC16E9.14c SPBP23A10.16 SPBP35G2.11c SPCC1235.11 SPCC23B6.05c * Data from SGD Gene name/description par1 uracil phosphoribosyltransferase serine/threonine protein kinase Sck2 serine/threonine protein kinase Gsk3, skp1 alpha-aminoadipate reductase transferase Lys7 urease accessory protein UreD nucleoside diphosphate kinase Ndk1 serine/threonine protein kinase Ksp1 prefoldin subunit 1 phosphatase activator transcription factor Gaf1 prevacuole/endosomal FYVE tethering component Pep7 short chain dehydrogenase 2-oxoglutarate dehydrogenase (lipoamide) L-azetidine-2-carboxylic acid acetyltransferase ppr1 succinate dehydrogenase Sdh1 sorting nexin Atg20 cytoskeletal protein binding protein Sla1 family, Shd1 mitochondrial coenzyme A transporter ubiquitin-protein ligase E4 DUF1640 family protein ornithine transaminase Car2 ChaC-like protein, predicted cation transport regulator sequence orphan glutathione synthetase large subunit Gsa1 transcription factor Moc3 RNA polymerase I transcription termination factor Reb1 heat shock protein Hsp104 cation diffusion family zinc membrane transporter Zrg17 TIM22 inner membrane protein import complex anchor subunit Tim18 transcription related zf-ZZ type zinc finger protein mitochondrial protein, human BRP44L ortholog DNA replication factor A subunit Ssb3 S. pombe CLS Increased Increased Increased Normal/Increased Increased Increased Increased Increased Decreased Increased Normal Decreased Normal Increased Increased Decreased Increased Increased Decreased Normal Increased Increased Normal Increased Normal Increased Increased Increased Increased Increased S. cerevisiae ortholog YOR014W YHR128W YHR205W YMR139W YGL154C NA YKL067W YHR082C YJL179W YOR043W YFL021W YDR323C YMR226C YIL125W YJL127C YKL148C YDL113C YBL007C YGR096W YDL190C YFL046W YLR438W YER163C NA YOL049W YDR034C YDR026C YLL026W YNR039C YDR178W S. cerevisiae CLS* NA NA Mixed outcome NA NA NA NA NA NA Decreased NA NA Increased NA Decreased NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA S. cerevisiae fitness* Decreased NA Decreased Decreased Decreased NA Increased Mixed outcome Decreased Decreased Increased Decreased Increased Decreased Decreased Normal Increased Decreased Decreased Decreased Decreased Increased Increased NA Decreased Decreased Decreased Decreased Decreased Decreased Increased Decreased Normal NA YGL080W NA NA NA NA Decreased NA YJL173C
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