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Lab 6. Skeletal and Smooth Muscle Jakoi 2014 SKELETAL MUSCLE VOCABULARY: Muscle fiber = cell, contains myofibrils (10-100 um) Myofibrils = made of filaments (1um) Myofilament = actin, myosin (20 nm) Fascicle = several fibers (cells) Epimysium- CT around several muscle fascicles Perimysium – CT around one muscle fascicle Endomysium – CT around single fiber (cell) TO IDENTIFY: Longitudinal Section: o Fibrous = very long cells (up to cms), 20-100 µm diameter o Striations = stripes = A-I banding, 2-4 µm long o Peripheral nuclei are cigar-shaped and dark staining o Multiple nuclei per cell Cross Section: o Large, polygonal cells, 20-100 µm diameter o Stippled appearance = 1-µm dots = myofibrils in cross section o Peripheral nuclei, dark staining Lab 6. Skeletal and Smooth Muscle Jakoi 2014 SMOOTH MUSCLE = postage stamp shape SMOOTH MUSCLE VERSUS SKELETAL MUSCLE SMOOTH MUSCLE VERSUS COLLAGEN