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From: PGC-1α Induces Human RPE Oxidative Metabolism and Antioxidant Capacity
Invest. Ophthalmol. Vis. Sci.. 2016;57(3):1038-1051. doi:10.1167/iovs.15-17758
Figure Legend:
Maturation of RPE in vitro is associated with increased mitochondrial mass. (A) Transepithelial resistance of ARPE-19
matured on Transwells for up to 4 weeks (n = 3 for each time point). (B) Gene expression of RPE-specific genes
measured by qPCR during ARPE-19 maturation (n = 3 for each time point). (C) MitoTracker CMTMRos staining
demonstrates increased mitochondrial network with maturation time. Images were acquired with variable exposure times
using a ×40 objective. Scale bar: 20 μm. (D) Quantification of image area covered by MitoTracker fluorescence as a
measure of mitochondrial network size. Area covered is significantly greater at 4 weeks compared with 70% confluent
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week confluent
(n = 9 for 70%The
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1 week and
= 6 for 4 in
weeks).
(E) MitoTracker
staining
images
acquired
with
Date
8/3/2017
fornResearch
Vision and
Ophthalmology
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© 2017.
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identical exposure times using a ×20 objective demonstrates increased fluorescence at longer maturation times. Values