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From: PGC-1α Induces Human RPE Oxidative Metabolism and Antioxidant Capacity Invest. Ophthalmol. Vis. Sci.. 2016;57(3):1038-1051. doi:10.1167/iovs.15-17758 Figure Legend: Maturation of RPE in vitro is associated with increased mitochondrial mass. (A) Transepithelial resistance of ARPE-19 matured on Transwells for up to 4 weeks (n = 3 for each time point). (B) Gene expression of RPE-specific genes measured by qPCR during ARPE-19 maturation (n = 3 for each time point). (C) MitoTracker CMTMRos staining demonstrates increased mitochondrial network with maturation time. Images were acquired with variable exposure times using a ×40 objective. Scale bar: 20 μm. (D) Quantification of image area covered by MitoTracker fluorescence as a measure of mitochondrial network size. Area covered is significantly greater at 4 weeks compared with 70% confluent andof1 download: week confluent (n = 9 for 70%The andAssociation 1 week and = 6 for 4 in weeks). (E) MitoTracker staining images acquired with Date 8/3/2017 fornResearch Vision and Ophthalmology Copyright © 2017. All rights reserved. identical exposure times using a ×20 objective demonstrates increased fluorescence at longer maturation times. Values