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S4 Table. Antibodies used for immunohistochemistry and immunofluorescence analysis.
Target
Antibody - Clone
Isotype
Immunogen
Purity
Source
Vitronectin
Mouse monoclonal [VIT-2]
to human vitronectin
IgM
Ascites fluid
Sigma
Mouse IgM
Goat anti-mouse IgM (μchain specific) conjugated
to horseradish peroxidase
Rabbit monoclonal
[EP873Y] to vitronectin
Purified human
plasma
vitronectin
Purified mouse IgM
Affinity isolated
antibody
Sigma
Tissue culture
supernatant
Abcam
Affinity-purified
antibodies that react
with IgG heavy chains
and all classes of
immunoglobulin light
chains from mouse
IgG fraction
Life Technologies
Protein G purified
Abcam
Ascites fluid
Sigma
Vitronectin
IgG1
Rabbit IgG
Goat anti-rabbit IgG
antibody, highly crossadsorbed and labeled with
Alexa Fluor 594
Cell membrane
marker
(Na+/K+ATPase)
Serous cell
marker
(Lactoferrin)
Submucosal
glands mucous
cell
Mouse monoclonal [464.6]
to Na+/K+-ATPase
IgG1
Mouse monoclonal (2B8) to
lactoferrin
IgG1
Mouse monoclonal [8C11]
to human MUC5B
IgG2a
Kappa
Synthetic peptide
corresponding to
residues near the V10
subunit of human
vitronectin
Full length native
protein purified from
rabbit renal outer
medulla
Full length human
native protein
(purified)
Mucin 5B partial (XP039877, 4186 - 
4296aa) recombinant
Abcam
(MUC5B)
Goblet cell
(MUC5AC)
Mouse monoclonal [45M1]
to mucin 5AC
Mouse IgG
Goat anti-mouse IgG
antibody, highly crossadsorbed and labeled with
Alexa Fluor 488
IgG1
with glutathione Stransferase tag
M1 mucin preparation
from the fluid of an
ovarian mucinous cyst
IgG fraction
Abcam
Affinity-purified
antibodies that react
with IgG heavy chains
and all classes of
immunoglobulin light
chains from mouse
Life Technologies
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