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Download 1 Supplemental Table III. T-DNA Mutant Screens, Genotyping and
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Supplemental Table III. T-DNA Mutant Screens, Genotyping and qPCR Primers Primer 201 208 244 LB 214 243 254 255 353 352 355 RB 353 352 357 358 360 361 355 LB 362 361 364 365 366 367 RGL2-3 RGL2-4 371 374 374 LB 374 373 378 379 Sequence 5’ CAGCTAAGCATCCGATTTGC 5’ TCACATAGAGAAGTCACATG 5’ ATGGC(G/T)GA(G/T)GTTGCT(T/C) (T/A)GAAACTCGA(A/G)CA 5' GGCAATCAGCTGTTGCCCGT CTCACTGGTG 5’ATGATCCATGGGACCTCTTCCGGCG AGTTCTAA 5’CGTCGAGAGTTTCCAAGCGGAGGTG GTAAT 5’ AGAAGCAATCCAGCAGA 5’ GTGTACTCTCTTCTTACCTTC 5’ GGTTCTTGGTGATGGAATCTCGA 5’ TTATTCCACACGATTGATTCGCCA 5’ ATGGC(A/T)GA(A/T/C)GT(A/T/G)GCA CA(G/C)AAG CTTGAACA 5’ TGATAGTGACCTTAGGCGACT TTTGAACGC see above 5’ CATCAATGACGACGGT 5’ GTACTCTGAGTCAGGCTT 5’ TGCGGTACCATGAAGAGAGGAT ACGGAG 5’ ATCGGTACCGCCGCGACTCAGG see above 5’GTTCTACTGTCTTAAACGACTCTGTT CATT see above 5’ CCAAAACCACTACCAGC 5’ TCCACACAACTTCGGG 5' ACCAAAACCACTACCAGC 5' GTCGTTTAAGACAGTAGAACCA 5' ACGCCCATGGAGATGAAGAGAGGA TACGGAGA 5' ACGCAGATCTCAGGCGAGTTTCCAC GCCGA 5’ GTTACATACACACATGTTCCTCGT TCTCGA 5’ ATTGCCTCTTCTAGTAATGCCTTC AATGAC see above see above 5’ATCGGTACCTTTATGACGACGTATC TAC 5’ CAATGATATTGCCTCTTCTAG 5’ CTGAGTCACACCAAGGA 1 Used for RGA genotyping rga-28 genotyping Notes 0.8 kb PCR product confirming absence of RGA transcript in rga-28 by RT-PCR 1.4 kb PCR product RGA qPCR RGL1 genotyping rgl1-2 genotyping 286 bp PCR product confirming absence of RGL1 transcript in rgl1-2 by RT-PCR RGL1 qPCR RGL2 genotyping 1.5 kb PCR product rgl2-13 genotyping confirming absence of RGL2 transcript in rgl2-13 by RT-PCR RGL2 qPCR (cDNA as a template) RGL2 qRT-PCR (RNA as a template) Gal4 AD-RGL2 1 kb PCR product RGL3 genotyping 1.5 kb PCR product rgl3-3 genotyping confirming absence of RGL3 transcript in rgl3-3 by RT-PCR RGL3 qPCR 0.3 kb PCR product 0.5 kb PCR product 1.5 kb PCR product 1.4 kb PCR product 260 bp PCR product 1.5 kb PCR product 1.4 kb PCR product 326 bp PCR product 220 bp PCR product 5' and 3' primers for amplifying RGL2 and cloning into pACTII 1.4 kb PCR product 224 bp PCR product 380 381 305 306 5'GA4BamHI 3'GA4SalI ubq11-3Frt ubq11-4Rrt ubq11Frt-2 ubq11Rrt-2 At18SFrt At18SRrt 5' AACCAGATCTCGATGAAACGAAG CCATCAA 5' AACTAGATCTTTATGACGACGTATC TAC 5’ CACACGACCGCTCATAG 5’ TGCCTATCCAATTTACCCTC 5'-CGCGGATCCGTGTTTAGAGGCCAT CCCATTCAC 5'-ACGCGTCGACCAAACAAATCATAT TGCTGAAATC 5’ GCAGATTTTCGTTAAAACC 5’ CCAAAGTTCTGCCGTCC 5'-CTTGGTTCTCAGGCTCC 5'-CAAATGAAGCGTAGACTCC 5'-ATACGTGCAACAAACCC 5'-CTACCTCCCCGTGTCA 2 Gal4 AD-RGL3 5' and 3' primers for amplifying RGL3 and cloning into pACTII GAI qPCR GA3ox1 RT-PCR (for checking genomic DNA contamination) 250 bp PCR product UBQ11 qPCR (cDNA as a template) UBQ11 qRT-PCR (RNA as a template) 18S rRNA qRT-PCR 167 bp PCR product genomic DNA: 1.6 kb PCR product cDNA: 1.1 kb PCR product 232 bp PCR product 301 bp PCR product
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