Download 赣 南 医 学 院 教 案

赣 南 医 学 院 教 案
教研室：Department of Biochemistry and Molecular Biology
课程名称
授课内容
Biochemistry
Chapter 10
授课专业
DNA Replication
教师姓名： Yan Yiming
Clinical medicine major
授课学时
6
【Master】
1. DNA semi-conservative replication.
2. The high fidelity of replication.
3. Process of DNA Replication.
4. Significance and type of gene mutation.
教学目的
【Understand】
1. Function of DNA polymerases, Primase, Topoisomerases and DNA
Ligase.
2. Formation of origin recognition and Okazaki Fragments.
3. Progress of Nucleotide Excision Repair and Reverse Transcription.
【Know】
1. The experimental evidences of DNA semi-conconservative replication.
2. Feature of Prokaryotic DNA Replication.
3. Repair of DNA damage.
1. DNA semi-conservative replication.
教学重点
2. The high fidelity of replication.
3. Process of DNA replication.
4. Significance and type of gene mutation.
教学难点
Process of DNA replication.
1. Theaching aid: computer, projector, mike, chalk, ferule, multimedia
教具和媒
体使用
courseware
2. Theaching means: utilize modernization teaching means and
methods, make good courseware, combine pictures with words well;
integrate languge expression, apporiate writing and teaching with
multimedia.
教学方法
The condition which needs by the DNA replication is a master line in
this lesson, and the DNA double helix structure knowledge which had
taught will be associated in the process, so the knowledge should be linked
up. We shall use the multimedia course wares fully, with the animation and
so on three-dimensional space structure drawing image, vividly teaches
DNA the biosynthesis process.
教学过程
Chapter 10 DNA Replication
Section 1 General features of DNA replication
1. Experiment of Semiconservative Replication
2. Significance of Semiconservative Replication
Section 2 Enzymes in DNA replication
1. Chemical Reaction of DNA Replication
2. DNA Polymerases
3. The unwinding in DNA Replication and topology change of DNA
4. Primase and Primosome
Section 3 Process of DNA replication
1. Origins of Replication
2. Elongation of DNA chains
3. Termination of DNA Replication
Section 4 Reverse transcriptionand others
Section 5 DNA damage and repair
1. Significance of Mutation
2. The Factor that initiate Mutation
3. Repair of DNA Damage
讲授新进展
内容
Telomere and Telomerase: Connection to Aging and Cancer.
参考书籍
思考题或/及
作业
课后总结
⑴ Berg J M，Tymoczko J L，Stryer L. 2002. Biochemistry. 5th
Edition. New York:W.H. Freeman Publishers.
⑵ Devlin T M.. 2002. Textbook of Biochemisrty with Clinical
Correlations. New York: Wiley-Liss.
⑶ Robert K. Murray, Daryl K. Granner, Peter A. Mayes，et al. 2003.
Harper’s Biochemistry. Stamford：McGraw-Hill.
1. After discussion with your group members, prepare a list of
compounds (e.g., ATP etc.) and enzymes that are needed for the replication
of DNA.
2. Discuss the functions of helicase (dnaB) and gyrase enzymes.
3. Discuss the process of replication and the role of dnaA, dnaB, dnaC,
SSB proteins in the replication process.
4. What functions do polymerase I, polymerase II, and polymerase III
enzymes play in replication process.
5. How lesions in DNA are repaired. Also explain how a thymine dimer
is removed from a DNA that has been exposed to UV light.
Each DNA strand serves as a template for the synthesis of a new strand,
producing two new DNA molecules, each with one new strand and one old
strand. This is semi-conservative replication. Process of DNA replication
includes three stages: initiation, elongation and termination. DNA
replication requires the following materials: substrate, template, enzyme,
primer, and other protein factors.