Download A New Method for Creating Transgenic Animals

A New Method for Creating Transgenic
Animals
INVENTORS • Robert Bremel, Anthony W. S. Chan, Jane Burns
WARF: P95253US
View U.S. Patent No. 6,080,912 in PDF format.
Since its founding in 1925 as the patenting and
licensing organization for the University of Wisconsin-
The Wisconsin Alumni Research Foundation (WARF) is seeking commercial
partners interested in developing improved methods for the production of
transgenic animals.
Madison, WARF has been working with business and
industry to transform university research into products
that benefit society. WARF intellectual property
managers and licensing staff members are leaders in
the field of university-based technology transfer. They
OVERVIEW
are familiar with the intricacies of patenting, have
worked with researchers in relevant disciplines,
understand industries and markets, and have
The ability to alter the genetic makeup of animals such as cows, pigs and sheep allows a
number of commercial applications. These applications include the production of animals
that express large quantities of exogenous proteins in their milk, are resistant to infection
or have enhanced growth rates.
negotiated innovative licensing strategies to meet the
individual needs of business clients.
The most widely used method for the production of transgenic animals is microinjection of
DNA into the pronuclei of fertilized embryos. However, while this method works well for the
production of transgenic mice, it is much less efficient for the production of transgenic
livestock.
THE INVENTION
UW–Madison researchers have developed improved methods and compositions for the
generation of transgenic non-human animals. The methods permit the introduction of
exogenous nucleic acid sequences into the genome of unfertilized eggs (e.g., prematuration oocytes and pre-fertilization oocytes) by microinjection of infectious retrovirus
into the perivitelline space of the egg. They increase the efficiency of producing transgenic
animals while reducing the rate of generating animals that are mosaic for the presence of
the transgene.
APPLICATIONS
• Production of transgenic animals, particularly cows
KEY BENEFITS
• Increased efficiency
Wisconsin Alumni Research Foundation | 614 Walnut Street, 13th Floor | Madison, WI 53726 | [email protected] | www.warf.org
WARF: P95253US
• Reduced generation of mosaic animals
ADDITIONAL INFORMATION
Tech Fields
Agriculture - Animal biotech
CONTACT INFORMATION
For current licensing status, please contact Emily Bauer at [email protected] or (608) 262-8638.
Wisconsin Alumni Research Foundation | 614 Walnut Street, 13th Floor | Madison, WI 53726 | [email protected] | www.warf.org