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Workshop-Module No. 21 Mass spectrometry: sample preparation, acquisition of data, computer-assisted data analysis and presentation Lecturers Length of course Teaching unit Language Capacity Prof. Dr. Volkmar Gieselmann, Dr. Marc Sylvester 3 days Institute of Biochemistry and Molecular Biology German or English (depending on participants) Maximum 6 participants Type of course Block course Lectures and guided computer work (CIP pool) Workload [h] 24 h (plus “homework”) Successful work with the exercises (i.e. computerassisted analysis of data set samples). Form of testing and examination Aims Contents Requirements for participation Recommended literature Remarks Achievement of a basic understanding in mass spectrometry. Training on ultra pure preparation of biological samples as a prerequisite for high quality data produced by highly sensitive analytical techniques. Basic knowledge on handling of mass spectrometry data and searches using different protein data bases. - Introduction into mass spectrometry and preparation of biological samples - Preparation of different biological samples for mass spectrometry - Measurement of biological samples by MALDI-TOF and ESI-Iontrap techniques - Identification of posttranslational modifications - Data analysis: - Identification of a protein by fingerprint analysis - Identification of a protein by protein sequencing - Identification of multiple proteins from a complex protein mixture Basic knowledge of biochemistry Selected publications in international journals Chapter “Mass spectrometry” in “Bioanalytik” by Lottspeich and Zorbas