Download Mass spectrometry: sample preparation, acquisition of data

Workshop-Module No. 21
Mass spectrometry: sample preparation, acquisition of data, computer-assisted
data analysis and presentation
Lecturers
Length of course
Teaching unit
Language
Capacity
Prof. Dr. Volkmar Gieselmann, Dr. Marc Sylvester
3 days
Institute of Biochemistry and Molecular Biology
German or English (depending on participants)
Maximum 6 participants
Type of course
Block course
Lectures and guided computer work (CIP pool)
Workload [h] 24 h (plus “homework”)
Successful work with the exercises (i.e. computerassisted analysis of data set samples).
Form of testing and examination
Aims
Contents
Requirements for participation
Recommended literature
Remarks
Achievement of a basic understanding in mass
spectrometry. Training on ultra pure preparation of
biological samples as a prerequisite for high quality data
produced by highly sensitive analytical techniques. Basic
knowledge on handling of mass spectrometry data and
searches using different protein data bases.
- Introduction into mass spectrometry and preparation
of biological samples
- Preparation of different biological samples for mass
spectrometry
- Measurement of biological samples by MALDI-TOF
and ESI-Iontrap techniques
- Identification of posttranslational modifications
- Data analysis:
- Identification of a protein by fingerprint analysis
- Identification of a protein by protein sequencing
- Identification of multiple proteins from a complex
protein mixture
Basic knowledge of biochemistry
Selected publications in international journals
Chapter “Mass spectrometry” in “Bioanalytik” by
Lottspeich and Zorbas