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MCF-7: transfection of siRNA VB-01LB-00, VB-01LB-01 24h scr 48h si 1 si 2 si 3 scr si 1 si 2 si 3 72h scr si 1 si 2 si 3 gene 1 alpha-tubulin Fig.1: Knock-down of gene expression in human breast adenocarcinoma MCF-7 cells after transfection of 3 different siRNA with Viromer® BLUE • • 25nM siRNA (standard protocol for adherent cells) good cell viability “We are very happy with the results. Everything is working fine and I nicely convinced my colleagues to change to Viromer as well. Thanks for good service! ” Dr. H. Jacobsson, Sahlgrenska University Hospital, Göteborg (Sweden) Lipocalyx GmbH Weinbergweg 23 06120 Halle Germany viromer-transfection.com viromer-transfection.com MCF-7: transfection of siRNA VB-siBUNDLE-01 Fig. 2: Monitoring of transfection complex uptake by human breast adenocarcinoma MCF-7 cells transfected with a Cy3-labeled siRNA using Viromer® BLUE Start Positive Controls (fluorescence microscopy). Internal data Lipocalyx GmbH Lipocalyx GmbH Weinbergweg 23 06120 Halle Germany viromer-transfection.com viromer-transfection.com Luciferase activity MCF-7: transfection of plasmid DNA Luciferase activity VR-01LB-00, VR-01LB-01 Fig.3: Comparative Luciferase activity after transfection of Renilla- and Firefly-Luciferase plasmids in human breast adenocarcinoma MCF-7 cells with Viromer® RED and 4 leading competitors Medical Faculty - University Halle (Germany) Lipocalyx GmbH Weinbergweg 23 06120 Halle Germany viromer-transfection.com viromer-transfection.com MCF-7: transfection of plasmid DNA VR-01LB-00, VR-01LB-01 Fig. 4: Transfection of human breast adenocarcinoma MCF-7 cells with FuGene® HD, Lipofectamine® 2000 and Viromer® RED Viromer® outperforms major competitors good cell viability Comparative data – generated internally at Lipocalyx GmbH Lipocalyx GmbH Weinbergweg 23 06120 Halle Germany viromer-transfection.com viromer-transfection.com MCF-7: transfection of plasmid DNA Fig. 5: Transfection of eGFP plasmid in human breast adenocarcinoma MCF-7 cells with Viromer® YELLOW VY-01LB-00, VY-01LB-01 And Start Positive® Controls VY-BUNDLE-01 Internal data Lipocalyx GmbH Lipocalyx GmbH Weinbergweg 23 06120 Halle Germany viromer-transfection.com viromer-transfection.com
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