Download Gene Forward primer (5`-3`) Reverse primer (5`

Table S1. Oligonucleotide primers and specific annealing temperatures for real-time RT-PCR.
Gene
Forward primer (5’-3’)
Reverse primer (5’-3’)
Collagen III
TTCCTTTTGTTCTAATCTTGTCA
TAGCACCATTGAGACATTTTGA
Collagen I
TGAGCCAGCAGATTGAGAAC
CCAGTGTCCATGTCGCAGA
VCAM-1
AGTCCCTCGTCCATCGTG
TGF-β1
Tm
60oC
PCR product (bp)
Reference
195
(1)
54oC
143
(1)
GAAAGAGGCTGTAGGTCC
60oC
122
(2)
AGGACGCCAACTTCTGCCT
AGGACCTTGCTGTACTGGGTGT
60oC
70
(3)
IL-10
GAGAACCACAGTCCAGCCAT
CATGGCTTTGTAGACGCCTT
60oC
179
(4)
MMP-9
CTTCCAACTTTGACAGCGACA
GGAGTGATCCAAGCCCAGTG
60oC
110
NM_00108220
3.1
IFN-γ
CTGGTCCAGCGTAAAGCAGT
TCAGTACTTGGATGCTCGCC
60oC
126
NM_00108199
1.1
TNF-α
AGATGGTCACCCTCAGATCAG
GAAGAGAACCTGGGAGTAGATGAG
60oC
206
(5)
MCP-1
CTTCTGTGCCTGCTGCTCATAG
TGCTTGGGGTCAGCACAGAT
60oC
221
NM_00108229
4.1
eNOS
GTGAGACTTTCTGCGTGGGA
CAGACCTGGCAGCAACTGTA
60oC
131
NM_00108273
3.1
TIMP-1
AGCAGAGCCTGCACCTGTGT
TGATTGACTTCTGGAGCCCC
60oC
101
(6)
GAPDH
GAACGGGAAACTCACTGGCAT
CCTTCTTGATGTCGTCATACTTAGC
54oC
110
(2)
VCAM: Vascular Cell Adhesion Molecule; TGF: Transforming Growth Factor; IL: Interleukin; MMP: Matrix Metalloproteinase; IFN: Interferon; TNF:
Tumor Necrosis Factor; MCP: Monocyte Chemoattractant Protein; eNOS: Endothelial Nitric Oxide Synthase; TIMP: TissueInhibitor of Metalloproteinase;
GAPDH: Glyceraldehyde 3-Phosphate Dehydrogenase; Tm: Annealing Temperature; RT-PCR:
Reverse Transcriptase-Polymerase Chain Reaction
Table –S1 References:
1. Dong B, Zhang C, Feng JB, Zhao YX, Li SY, Yang YP, Dong QL, Deng BP, Zhu L, Yu QT, Liu CX, Liu B, Pan CM, Song HD, Zhang MX, Zhang Y.
Overexpression of ACE2 enhances plaque stability in a rabbit model of atherosclerosis. Arterioscler Thromb Vasc Biol. 2008 Jul;28(7):1270-6.
2. Li CJ, Sun HW, Zhu FL, Chen L, Rong YY, Zhang Y, Zhang M. Local adiponectin treatment reduces atherosclerotic plaque size in rabbits. J Endocrinol.
2007 Apr;193(1):137-45.
3. Hofstaetter JG, Wunderlich L, Samuel RE, Saad FA, Choi YH, Glimcher MJ. Systemic hypoxia alters gene expression levels of structural proteins and
growth factors in knee joint cartilage. Biochem Biophys Res Commun. 2005 May 6;330(2):386-94.
4. Godornes C, Leader BT, Molini BJ, Centurion-Lara A, Lukehart SA. Quantitation of rabbit cytokine mRNA by real-time RT-PCR. Cytokine. 2007
Apr;38(1):1-7.
5. Charoenwanthanang P, Lawanprasert S, Phivthong-Ngam L, Piyachaturawat P, Sanvarinda Y, Porntadavity S. Effects of Curcuma comosa on the
expression of atherosclerosis-related cytokine genes in rabbits fed a high-cholesterol diet. J Ethnopharmacol. 2011 Apr 12;134(3):608-13.
6. Park KC, Park EJ, Kim ER, Kim Y, Chung SH, Cho BW, Kim S, Jin M. Therapeutic effects of PG201, an ethanol extract from herbs, through cartilage
protection on collagenase-induced arthritis in rabbits. Biochem Biophys Res Commun. 2005 Jun 17;331(4):1469-77.