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Supplemental Figure S8. Molecular analysis of the candidate gene CmKFB. A, CmKFB digital
expression (RPKM) pattern of ‘white’ and ‘yellow’ bulks at three fruit developmental stages. B,
The 12-bp deletion found in the CmKFB 5'-UTR of ‘TVT’ (the non-flavonoid accumulating
parent). C, Agarose gel fractionation of PCR amplification of DNA fragments containing the 5’UTR deletion performed on 14 melon accessions in addition to ‘NA’ and ‘TVT’. NarCh
accumulating varieties are indicated in yellow. D, Relative expression analysis of CmKFB by
qRT-PCR in fruits and in leaves of yellow (Y-F3) and white (W-F3) F3 bulks.
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