Download Powerpoint - Circulation Research

Procoagulant Soluble Tissue Factor Is Released From
Endothelial Cells in Response to Inflammatory Cytokines
by Björn Szotowski, Silvio Antoniak, Wolfgang Poller, Heinz-Peter Schultheiss, and
Ursula Rauch
Circulation Research
Volume 96(12):1233-1239
June 24, 2005
Copyright © American Heart Association, Inc. All rights reserved.
Figure 1. Induction of asHTF and full-length TF mRNA expression in HUVECs in response to
TNF-α (A and C) and IL-6 (B and D) stimulation. +P<0.0005 vs 0 minutes, *P<0.005 vs 0 minutes,
‡P<0.003 versus 0 minutes, ¥P<0.001 vs 0 minutes, #P<0.01 vs 0 minutes, and $P<0.05 vs 0
minutes.
Björn Szotowski et al. Circ Res. 2005;96:1233-1239
Copyright © American Heart Association, Inc. All rights reserved.
Figure 2. Cytokine-induced asHTF expression in HUVECs. Negative controls (A and B).
Björn Szotowski et al. Circ Res. 2005;96:1233-1239
Copyright © American Heart Association, Inc. All rights reserved.
Figure 3. asHTF and full-length TF protein expression on cytokine stimulation: Western blot
analysis of cell lysates (A) and cell culture supernatant (B) detected with a polyclonal antibody
against the extracellular domain of TF. A, lane 1: TNF-α stimulated for 5 hours; lane 2: TNF-α
stimulated for 18 hours; lane 3: control; lane 4: IL-6 stimulated for 5 hours; lane 5: IL-6
stimulated for 18 hours.
Björn Szotowski et al. Circ Res. 2005;96:1233-1239
Copyright © American Heart Association, Inc. All rights reserved.
Figure 4. Redistribution of cellular TFPI to the supernatant due to cytokine stimulation: Western
blot analysis of cell lysates (A) and the corresponding supernatants (B) of unstimulated and
stimulated HUVECs detected with a polyclonal antibody directed against TFPI. Lane 1:
unstimulated control; lane 2: TNF-α treatment for 5 hours; lane 3: TNF-α treatment for 18 hours;
lane 4: IL-6 treatment for 18 hours; lane 5: IL-6 treatment for 5 hours; lane 6: unstimulated
control.
Björn Szotowski et al. Circ Res. 2005;96:1233-1239
Copyright © American Heart Association, Inc. All rights reserved.
Figure 5. Cytokine-induced TF expression and TFPI reduction/secretion in HUVECs. Fluorescent
images of cellular TF (green), TFPI (red), and the nucleus (blue) in HUVECs before (A through C)
and after 5 hour TNF-α (D through F) or IL-6 (G through I) stimulation.
Björn Szotowski et al. Circ Res. 2005;96:1233-1239
Copyright © American Heart Association, Inc. All rights reserved.
Figure 6. Procoagulant activity of cells (A and B), cell supernatant (C through E), and MPs (F) in
response to stimulation with TNF-α (A, C, D, and F) and IL-6 (B and E). •P<0.00001, *P<0.0002,
†P<0.0003, +P<0.0004, #P<0.001, ±P<0.002, ‡P<0.003, §P<0.01, ¥P<0.03, and ¶P<0.05 TF activity
of unstimulated (solid-filled bar) and cytokine-stimulated (open bar) HUVECs at different points
in time (A for TNF-α, B for IL-6).
Björn Szotowski et al. Circ Res. 2005;96:1233-1239
Copyright © American Heart Association, Inc. All rights reserved.
Figure 7. Detection of MPs present in the extracellular fluid of endothelial cells after stimulation
with TNF-α for 5 hours.
Björn Szotowski et al. Circ Res. 2005;96:1233-1239
Copyright © American Heart Association, Inc. All rights reserved.