Download Supplemental Table 4

Gene Name
ARHGDIB (Rho GDP
Dissociation Inhibitor
(GDI) β
B3GNT1 (UDPGlcNAc:βGal β-1,3-Nacetylglucosaminyltransferase 1)
B4GALT1 (UDPGal:βGlcNAc β 1,4galactosyltransferase,
polypeptide 1)
CTNNB1
(catenin, beta 1)
CUX1 (cut-like
homeobox 1)
EIF3A (eukaryotic
translation initiation
factor 3, subunit A)
FADD (Fas associated
via death domain)
FSCN1 (fascin actinbundling protein 1)
HEXA
(hexosaminidase A)
PCBP2 (poly(rC) binding
protein 2)
PDIA3 (protein
disulfide isomerase
family A, member 3)
POLR2A (polymerase II
polypeptide A)
S100A4 (S100 calcium
binding protein A4)
SDC1 (syndecan 1)
SRSF2 (serine/argininerich splicing factor 2)
STIP1 (stress-induced
phosphoprotein 1)
TCEB1 (transcription
elongation factor B)
TGF 1 (transforming
growth factor, beta 1)
Effects on
Tumor
Ref.
Both
Log2 Fold Change
O vs M
O vs Y
O vs A
M vs Y
M vs A
Y vs A
(1,
2)
-0.6541 -0.4321 -0.5128
0.2220
0.1414
-0.0807
Inhibits
(3)
0.6048
-0.1637 -0.2436
-0.0799
Promotes
(4)
-0.3074 -0.4193 -0.4038 -0.1119 -0.0964
0.0155
Promotes
(5)
-0.5703 -0.8412 -0.3220 -0.2709
0.2483
0.5192
Inhibits
(6)
-0.5483 -0.5855 -0.3024 -0.0371
0.2459
0.2831
Promotes
(7)
-0.6112 -0.6778 -0.4621 -0.0666
0.1491
0.2157
Promotes
(8)
0.4312
0.3442
0.1014
-0.0870
-0.1884
Promotes
(9)
-1.4195 -1.0807 -0.3833
0.3388
1.0362
0.6974
Promotes
(10,
11)
-0.7743 -0.5425 -0.2717
0.2318
0.5026
0.2708
Inhibits
(12)
-0.7270 -0.6081 -0.3711
0.1189
0.3559
0.2370
Promotes
(13)
-1.4168 -0.9836 -0.3972
0.4332
1.0197
0.5865
Promotes
(14)
-0.6701 -0.5241 -0.3379
0.1460
0.3322
0.1862
Promotes
(15)
-0.5497 -0.9786 -0.2846 -0.4289
0.2652
0.6941
Promotes
(16)
-0.6220 -0.4994 -0.4557
0.1664
0.0437
Promotes
(17)
-0.3823 -0.8971 -0.6749 -0.5148 -0.2926
0.2222
Promotes
(18)
-0.5598 -0.6239 -0.6151 -0.0641 -0.0553
0.0088
Promotes
(19)
0.3450
0.1935
-0.0467
-0.2402
Promotes
(20)
-0.5532 -0.7938 -0.4962 -0.2406
0.0570
0.2976
0.4411
0.5326
0.5386
0.3612
0.2984
0.1226
Supplemental Table 4. Eighteen key genes involved in age-dependent tumor progression.
These 18 genes were determined by finding the common genes for old tumor samples (O)
versus all other ages (Adolescent (A), Young Adult (YA), and Middle-Aged (MA)) that are in
common between the significant upstream regulators and the biofunction analysis. The second
and third columns denote the effects these genes have on tumor progression based on the
literature. The genes in bold and italic are found to overlap with genes found in the Gene Set
Enrichment Analysis (GSEA) gene sets for old tumor samples vs other aged tumor samples.
1.
Li X, Wang J, Zhang X, Zeng Y, Liang L, Ding Y. Overexpression of RhoGDI2 correlates
with tumor progression and poor prognosis in colorectal carcinoma. Ann Surg Oncol.
2012;19:145-53.
2.
Said N, Sanchez-Carbayo M, Smith SC, Theodorescu D. RhoGDI2 suppresses lung
metastasis in mice by reducing tumor versican expression and macrophage infiltration. J Clin
Invest. 2012;122:1503-18.
3.
Lee SH, Hatakeyama S, Yu SY, Bao X, Ohyama C, Khoo KH, et al. Core3 O-glycan
synthase suppresses tumor formation and metastasis of prostate carcinoma PC3 and LNCaP
cells through down-regulation of alpha2beta1 integrin complex. J Biol Chem. 2009;284:1715769.
4.
Choi HJ, Chung TW, Kim CH, Jeong HS, Joo M, Youn B, et al. Estrogen induced beta-
1,4-galactosyltransferase 1 expression regulates proliferation of human breast cancer MCF-7
cells. Biochem Biophys Res Commun. 2012;426:620-5.
5.
King TJ, Gurley KE, Prunty J, Shin JL, Kemp CJ, Lampe PD. Deficiency in the gap
junction protein connexin32 alters p27Kip1 tumor suppression and MAPK activation in a tissuespecific manner. Oncogene. 2005;24:1718-26.
6.
McNerney ME, Brown CD, Wang X, Bartom ET, Karmakar S, Bandlamudi C, et al. CUX1
is a haploinsufficient tumor suppressor gene on chromosome 7 frequently inactivated in acute
myeloid leukemia. Blood. 2012.
7.
Saletta F, Suryo Rahmanto Y, Richardson DR. The translational regulator eIF3a: the
tricky eIF3 subunit! Biochim Biophys Acta. 2010;1806:275-86.
8.
Tourneur L, Buzyn A, Chiocchia G. FADD adaptor in cancer. Med Immunol. 2005;4:1.
9.
Al-Alwan M, Olabi S, Ghebeh H, Barhoush E, Tulbah A, Al-Tweigeri T, et al. Fascin is a
key regulator of breast cancer invasion that acts via the modification of metastasis-associated
molecules. PLoS One. 2011;6:e27339.
10.
Pasztoi M, Nagy G, Geher P, Lakatos T, Toth K, Wellinger K, et al. Gene expression and
activity of cartilage degrading glycosidases in human rheumatoid arthritis and osteoarthritis
synovial fibroblasts. Arthritis Res Ther. 2009;11:R68.
11.
Jiang SS, Chen CH, Tseng KY, Tsai FY, Wang MJ, Chang IS, et al. Gene expression
profiling suggests a pathological role of human bone marrow-derived mesenchymal stem cells
in aging-related skeletal diseases. Aging (Albany NY). 2011;3:672-84.
12.
Roychoudhury P, Paul RR, Chowdhury R, Chaudhuri K. HnRNP E2 is downregulated in
human oral cancer cells and the overexpression of hnRNP E2 induces apoptosis. Mol Carcinog.
2007;46:198-207.
13.
Pressinotti NC, Klocker H, Schafer G, Luu VD, Ruschhaupt M, Kuner R, et al. Differential
expression of apoptotic genes PDIA3 and MAP3K5 distinguishes between low- and high-risk
prostate cancer. Mol Cancer. 2009;8:130.
14.
Saviozzi S, Cordero F, Lo Iacono M, Novello S, Scagliotti GV, Calogero RA. Selection of
suitable reference genes for accurate normalization of gene expression profile studies in nonsmall cell lung cancer. BMC Cancer. 2006;6:200.
15.
Yan XL, Jia YL, Chen L, Zeng Q, Zhou JN, Fu CJ, et al. Hepatocellular carcinoma-
associated mesenchymal stem cells promote hepatocarcinoma progression: Role of the
S100A4-miR155-SOCS1-MMP9 axis. Hepatology. 2013.
16.
Goodison S, Chang M, Dai Y, Urquidi V, Rosser CJ. A multi-analyte assay for the non-
invasive detection of bladder cancer. PLoS One. 2012;7:e47469.
17.
Edmond V, Brambilla C, Brambilla E, Gazzeri S, Eymin B. SRSF2 is required for sodium
butyrate-mediated p21WAF1 induction and premature senescence in human lung carcinoma
cell lines. Cell Cycle. 2011;10:1968-77.
18.
Wang TH, Chao A, Tsai CL, Chang CL, Chen SH, Lee YS, et al. Stress-induced
phosphoprotein 1 as a secreted biomarker for human ovarian cancer promotes cancer cell
proliferation. Mol Cell Proteomics. 2010;9:1873-84.
19.
Jalava SE, Porkka KP, Rauhala HE, Isotalo J, Tammela TL, Visakorpi T. TCEB1
promotes invasion of prostate cancer cells. Int J Cancer. 2009;124:95-102.
20.
Nguyen DH, Martinez-Ruiz H, Barcellos-Hoff MH. Consequences of epithelial or stromal
TGFbeta1 depletion in the mammary gland. J Mammary Gland Biol Neoplasia. 2011;16:147-55.