Download Powerpoint - Circulation Research

Apoptosis of Rat Vascular Smooth Muscle Cells Is
Regulated by p53-Dependent and -Independent Pathways
by M. R. Bennett, G. I. Evan, and S. M. Schwartz
Circulation Research
Volume 77(2):266-273
August 1, 1995
Copyright © American Heart Association, Inc. All rights reserved.
Time-lapse videomicrograph of rat vascular smooth muscle cells infected with c-myc showing
evidence of cell shrinkage, membrane blebbing (small arrow), and formation of an apoptotic
body (large arrow).
M. R. Bennett et al. Circ Res. 1995;77:266-273
Copyright © American Heart Association, Inc. All rights reserved.
Electron micrograph of rat VSMCs undergoing apoptosis.
M. R. Bennett et al. Circ Res. 1995;77:266-273
Copyright © American Heart Association, Inc. All rights reserved.
Cumulative number of apoptotic deaths per 100 cells over 24 hours for normal rat VSMCs and
cells infected with c-myc, bcl-2, p53 (wild-type conformation), E1A, or the retrovirus vector alone
in medium containing 0% FCS. Values are mean±SEM.
M. R. Bennett et al. Circ Res. 1995;77:266-273
Copyright © American Heart Association, Inc. All rights reserved.
Cumulative number of apoptotic deaths per 100 cells over 24 hours for rat VSMCs infected with
E1A or c-myc with and without bcl-2 in medium containing 0% FCS. Values are mean±SEM.
M. R. Bennett et al. Circ Res. 1995;77:266-273
Copyright © American Heart Association, Inc. All rights reserved.
Northern hybridization of normal rat VSMCs or cells infected with c-myc, E1A, bcl-2, or p53
genes probed for c-myc, p53, and bcl-2 mRNA.
M. R. Bennett et al. Circ Res. 1995;77:266-273
Copyright © American Heart Association, Inc. All rights reserved.
Western blot of lysates from normal rat VSMCs and cells infected with c-myc, bcl-2, E1A, or p53
genes probed for p53 protein.
M. R. Bennett et al. Circ Res. 1995;77:266-273
Copyright © American Heart Association, Inc. All rights reserved.