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Inhibition of antithrombin by Plasmodium falciparum histidine-rich protein II by Matthew Ndonwi, Oname O. Burlingame, Aaron S. Miller, Douglas M. Tollefsen, George J. Broze, and Daniel E. Goldberg Blood Volume 117(23):6347-6354 June 9, 2011 ©2011 by American Society of Hematology SDS-PAGE and Western blot analysis of purified HRPII. (A) SDS-PAGE. Matthew Ndonwi et al. Blood 2011;117:6347-6354 ©2011 by American Society of Hematology Interaction of HRPII with GAGs. (A) Binding of HRPII to immobilized heparin. Matthew Ndonwi et al. Blood 2011;117:6347-6354 ©2011 by American Society of Hematology Effect of HRPII on the AT-heparin inhibition of FXa. Reaction buffer alone as control or reaction buffer containing indicated combinations of heparin (0.01 U/mL), AT (100nM), and HRPII (300nM) was added to a fixed amount of FXa (5nM). Matthew Ndonwi et al. Blood 2011;117:6347-6354 ©2011 by American Society of Hematology Effect of various divalent cations on the reversal of the AT-heparin inhibition of FXa by HRPII. (A) Effect of various cations and Zn2+ counterions. Matthew Ndonwi et al. Blood 2011;117:6347-6354 ©2011 by American Society of Hematology IC50 for HRPII reversal of AT-heparin and HCII-heparin activities. Matthew Ndonwi et al. Blood 2011;117:6347-6354 ©2011 by American Society of Hematology Effect of HRPII on the heparin-induced prolongation of plasma clot time. aPTT reagent (100 μL) was premixed with citrated pooled normal plasma (100 μL) before adding zinc acetate (10 μL, 20 μM final) with or without varied concentrations of HRPII (20μL) wit... Matthew Ndonwi et al. Blood 2011;117:6347-6354 ©2011 by American Society of Hematology
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