Download Center for Inherited Neuromuscular Disease

Wolfson Centre for Inherited Neuromuscular Disease
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Product Datasheet for
Monoclonal Antibody
Name: MANEX1216B - MOUSE ANTI-DYSTROPHIN
Clone No. 6B11
Specificity: Dystrophin Western blots only
Species: human, mouse, xenopus .
Epitope: exons 12-16
Isotype: IgG2a
Western blot: Stains a 427kD band in normal human muscle extract. Dilution: 1/100
dystrophin
Human muscle extract.
Normal human muscle section
Immunolocalization: Stains extracellular material in muscle sections, without clear membrane
staining.
Dilution: 1/4
Immunogen: Recombinant human dystrophin fragment encoded by exons 4-16.
Storage recommendation: Store one month's use at 4oC and remainder in aliquots at –20oC.
Stable for at least 12 months under these conditions
Concentration: estimated at 40micrograms per ml (not measured directly)
Supplied as liquid culture supernatant in DMEM/10% horse serum (contains AZIDE: toxic!)
(Identical culture medium can be supplied as negative control)
Reference in: In vivo targeted repair of a point mutation in the canine dystrophin gene
by a chimeric RNA/DNA oligonucleotide. R.J. Bartlett, S. Stockinger, M.M. Denis,
W.T. Bartlett, L. Inverardi, T.T. Le, Nguyen thi Man, G.E. Morris, D.J. Bogan, J.
Metcalf-Brogan and J.N. Kornegay . Nature Biotechnol. 18 (2000) 615-622