Download Nuclear membrane Clone AE-5 Cat. no. MON 7061

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Nuclear membrane
Clone AE-5
Cat. no. MON 7061
Specificity
AE-5 recognizes an antigen associated with the nuclear membrane expressed in human cells.
Source: A BALB/c mouse was immunized with nuclei of myeloid leukemia biopsy cells. The
isolated splenocytes were fused with mouse myeloma cells.
Immunoglobulin type
Mouse IgG1
Use
AE-5 produces a ring staining pattern around the nucleus of normal and malignant cells and can
be used for paraformaldehyde fixed or frozen tissue or cell preparations and formalin fixed,
paraffin-embedded tissue sections. Tonsil tissue is the recommended positive control tissue. The
mAb can also be used as a marker of the nuclear membrane in subcellular fractions.
frozen
sections
+
paraffin
sections
+
flow
cytometry
+
ELISA
n.d.
blocking
Western
blotting
n.d.
n.d.
Presentation
100 µg purified material in PBS with 0.05% sodium azide
Concentration: 100 ug/ml.
Literature
This clone has not been published but similarly generated and characterized clones are described
in:
- Epstein, A.L. and Clevenger, C.V., Identification of nuclear antigens in human cells by
immunofluorescence, immunoelectron microscopy, and immuno-biochemical methods using
monoclonal antibodies. In: Progress on nonhistone protein research, Vol. 1, Isaac Bekhor, ed.,
1985, CRC Press, Boca Raton, FL, pp 117-137.
FOR RESEARCH USE ONLY, NOT FOR DRUG, DIAGNOSTIC OR OTHER USE.
MONOSAN, Brandname of SANBIO B.V.
P.O.Box 540, 5400 AM UDEN, The Netherlands
Tel. +31 413 251115, Fax. +31 413 266605
E-mail : [email protected]
11/08 Cat.# MON 7061