Download Two-dimensional Gel Electrophoresis

SAP#__________________
Invoice #__________________
INVOICE
Gene Expression
Center for Biotechnology, University of Nebraska-Lincoln
The Beadle Center E243, 19th & Vine Street, Lincoln, NE 68588-0666
Gene expression personnel:
Center for Biotechnology:
User Information
User Name:
P.I. Name:
Department:
Address:
Phone: (402) 472-8969 (lab), Email: [email protected]
Phone: (402) 472-2635, Fax (402) 472-3139
Date:
Phone:
Debit#:
Vector construction (E. coli)
□ Expression vector construction, specify tag ($100)
□ Gene modification (fusion to expression signals)
standard PCR ($10); overlap-extension (OLE) PCR ($50)
□ Screening of transformants/restriction analysis ($50)
□ Transformation ($25)
□ Plasmid preparation, mini, midi and maxi preps ($8, $30, $50)
□ Site directed mutagenesis (inquire)
□ other – describe - _________________________________________
$
Strain construction (E. coli and S. solfataricus)
□ Gene disruption (inquire)
□ Essential gene test (inquire)
□ Regulated in vivo expression (inquire)
□ Gene deletion, substitution mutations (inquire)
□ other – describe - _________________________________________
$
Protein overproduction (E. coli and S. solfataricus)
□ Gene induction/protein abundance (solubility) analysis by coomassie ($100)
by western ($100)
□ Fermentation optimization ($100)
□ Codon optimization (inquire)
□ mRNA stabilization (inquire)
□ Protein secretion (inquire)
□ Protein co-expression (inquire)
□ Protein folding--in vivo-chaperones (inquire)
□ Protein folding--in vitro (inquire)
□ other – describe - _________________________________________
TOTAL
Special Instructions:
$
$