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Identification of Four Genes in Endothelial Cells Whose Expression Is Affected by Tumor Cells and Host Immune Status—A Study in Ex Vivo–Isolated Endothelial Cells by Birgit Liliensiek, Marian Rocha, Victor Umansky, Axel Benner, Jie Lin, Reinhard Ziegler, Peter P. Nawroth, and Volker Schirrmacher Blood Volume 92(9):3394-3404 November 1, 1998 ©1998 by American Society of Hematology Analysis of the effect of tumor growth on the expression of multiple genes in liver sinusoidal endothelial cells in vivo. Birgit Liliensiek et al. Blood 1998;92:3394-3404 ©1998 by American Society of Hematology TNF- and tumor cell supernatant exert suppressive effects in vitro on the expression of selected genes by BAEC. Confluent BAEC were cultured for 8 hours either without (C) or with 1 nmol/L TNF- (TNF) and/or with lymphoma cell conditioned supernatant (TS). Birgit Liliensiek et al. Blood 1998;92:3394-3404 ©1998 by American Society of Hematology Downregulation of gene expression in ex vivo–isolated liver endothelial cells from tumorbearing immuno-competent mice. Birgit Liliensiek et al. Blood 1998;92:3394-3404 ©1998 by American Society of Hematology Upregulation of gene expression in ex vivo–isolated liver endothelial cells from tumor-bearing immuno-incompetent mice. Birgit Liliensiek et al. Blood 1998;92:3394-3404 ©1998 by American Society of Hematology Supernatants of tumor and sinusoidal cells isolated from metastatic livers of immuno-competent DBA/2 and immuno-incompetent Balb/c (nu/nu) mice at a late stage of tumor growth alter gene expression in BAEC. Supernatants of the re-isolated and cultured cells... Birgit Liliensiek et al. Blood 1998;92:3394-3404 ©1998 by American Society of Hematology Gene expression of A4, B2, C3, and E10 in cultured bovine capillary endothelial cells after TNFand tumor cell supernatant is different from BAEC. Confluent ACE were cultured for 8 hours either without (C) or with 1 nmol/L TNF- (TNF) and/or with lymphoma... Birgit Liliensiek et al. Blood 1998;92:3394-3404 ©1998 by American Society of Hematology Birgit Liliensiek et al. Blood 1998;92:3394-3404 ©1998 by American Society of Hematology Birgit Liliensiek et al. Blood 1998;92:3394-3404 ©1998 by American Society of Hematology Birgit Liliensiek et al. Blood 1998;92:3394-3404 ©1998 by American Society of Hematology