Download KERIMAN SENER

Keriman Livingston
Richland College Department of Biology
School of Mathematics, Science and Health Professions
ACCESS office, A-110, 972-238-6140, [email protected]
EDUCATION
Ph.D. in Biochemistry
Northeastern University, Boston, MA (May 2006)
Advisor: Prof. Edward L. Jarroll
Dissertation: “UDP-N-Acetylglucosamine Pyrophosphorylase and Quantification of Sugar
Phosphate Intermediates in the Encystment Pathway of Giardia intestinalis”
M.S. in Molecular Biology
Northeastern University, Boston, MA, (May 2000)
Advisor: Prof. Jacqueline Piret
Thesis: “Molecular Analysis of Developmental Genes in Streptomyces coelicolor
and Streptomyces lividans”
B.S. in Biology
Middle East Technical University, Ankara, Turkey (June 1994)
RESEARCH EXPERIENCE
Ph.D. Dissertation
Carried the enzymatic characterization of the recombinant UDP-GlcNAc pyrophosphorylase in
intestinal parasite Giardia intestinalis.
Developed a capillary electrophoretic methodology for the separation, detection and
quantification of sugar phosphate intermediates of the encystment pathway of Giardia intestinalis.
Summer Internship
OraVax Inc (currently Acambis), Cambridge MA (2000)
Advisor: Dr. Thomas Ermak
Investigated the roles of cell and antibody-mediated immunity in protection against
Helicobacter pylori infection in mice immunized with recombinant Helicobacter pylori urease vaccine.
M.S. Thesis
Using gene replacements, determined the possible function(s) of the several genes in
morphological development and antibiotic production in Streptomyces coelicolor and Streptomyces
lividans.
TECHNICAL SKILLS
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Axenic cultivation of Streptomyces coelicolor, Streptomyces lividans, Escherichia coli and Giardia
intestinalis cells
Genetic transformation and gene cloning in Streptomyces and Escherichia coli
Genomic and plasmid DNA isolation, protoplast formation and regeneration and Southern blotting
Assays of antibiotic production
Expression and purification of recombinant proteins in Escherichia coli, SDS-PAGE
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Antibody purification, immunoprecipitation, immunohistochemical staining, Western blotting and
ELISA
Separation and quantification of amino sugar phosphates by capillary electrophoresis
Radioactive (using a phosphoimager) and colorimetric enzyme assays and determination of
enzyme kinetic parameters
Thin layer chromatography
Analysis of DNA sequences and protein structures
Microsoft Office Suite (PowerPoint, Excell, Word), Adobe Photoshop
AWARDS
Conference Award by Graduate and Professional Student Association
Northeastern University (Summer 2001)
Full scholarship for M.S. and Ph.D. studies at Northeastern University
Turkish Council of Higher Education, Ankara, Turkey (1996-1998)
Class Salutatorian in Faculty Graduation
Middle East Technical University, Department of Biology (1994)
Dr. Olcay Birgul Scholarship for honor students
Middle East Technical University, Department of Biology (1994)
Dean’s list during seven semesters
Middle East Technical University, Department of Biology (1990-1994)
TEACHING EXPERIENCE
Adjunct Faculty in General Biology
Richland College, Department of Mathematics, Science and Health Professions
Dallas, TX (2008-2011)
Lecturer in General Biology
Northeastern University, Department of Biology
Boston, MA (2005, half semester)
Laboratory Lecturer in Microbiology
Northeastern University, Division of Adult and Continuing Education
Boston, MA (2004-2005)
Teaching Assistant in Microbiology and General Biology
Northeastern University, Department of Biology
Boston, MA (2004-2006, 1998-2000)
Teaching Assistant in General Biology, Botany and Invertebrates
Mustafa Kemal University, Department of Biology
Hatay, Turkey (1995-1996)
Teaching Assistant in Physiology and Genetics
Middle East Technical University, Department of Biology
Ankara, Turkey (1994-1995)
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PUBLICATIONS
Publications in International Journals and Books
Sener, K., van Keulen, H. and Jarroll, E. L. 2009. Giardan:Synthesis, Regulation and Inhibition.
In:Giardia and Cryptosporidium from Molecules to Disease. Eds: Ortega-Pierras, G., Caccio, S.,
Foyer, R., Mauk, T., Smith, H., and Thompson, A. CABI International, Chambridge, MA.
pp 382-397.
Lopez, A. B., Sener, K., Trosien, J., Jarroll, E. L., and van Keulen H. 2007. UDP-N-Acetylglucosamine
4’-Epimerase from the Intestinal Protozoan Giardia intestinalis Lacks UDP-Glucose 4’-Epimerase
Activity. Journal of Eukaryotic Microbiology. 54:154-160.
Sener, K., Shen, Z., Newburg, D., and Jarroll, E. L. 2004. Amino sugar phosphate levels in Giardia
change during cyst wall formation. Microbiology. 150:1225-1230.
Jarroll, E. L. and Sener, K. 2003. Potential drug targets in cyst wall biosynthesis by intestinal
protozoa. Drugs Resistance Updates. 6:239-246.
Lopez, A. B., Sener, K., Jarroll E. L., and van Keulen, H. 2003. Transcription regulation is
demonstrated for five key enzymes in Giardia intestinalis cyst wall polysaccharide biosynthesis.
Molecular and Biochemical Parasitology. 128:51-57.
Publications in Conference Proceedings
Jarroll, E. L., Sener, K., and van Keulen, H. Characterization of an inducible UDP-Nacetylglucosamine pyrophosphorylase in Giardia intestinalis. 12th International Congress of
Parasitology. Guangzhou, China. July, 2005.
Sener, K., Shen, Z., Newburg, D., and Jarroll E. L. Levels of sugar phosphate intermediates in Giardia
change during encytment. 8th International Workshops on Opportunistic Protists (IWOP-8) and
International Conference on Anaerobic Protists (ICAP). Hilo, Hawaii. July, 2003.
Newburg, D., Sener, K., Shen, Z., Jacewicz, M. S., and Jarroll, E. L. Quantification of aminosugar
phosphates by capillary electrophoresis. FASEB J 2003;17:A164. Experimental Biology 2003, San
Diego, CA. April, 2003.
Sener, K., Jarroll, E. L., Lopez, A. B., and van Keulen, H. UDP-GlcNAc pyrophosphorylases in
Giardia. 10th International Congress of Parasitology. Vancouver, Canada. August, 2002.
Jarroll, E. L., Lopez, A. B., van Keulen, H., Trosien, J., Sener, K., and Argyros, F. UDP-GlcNAc 4'epimerase is a second inducible enzyme in Giardia intestinalis encystment. 11th International
Congress of Protozoology. Salzburg, Austria. July, 2001.
Kazi, L., Sener, K., Feng, F., and Piret, J. M. Gene disruptions in the Streptomyces coelicolor
developmental region bldB and Properties of the BldB Protein. Abstract P23. Genetics and Molecular
Biology of Industrial Microorganisms. Bloomington, IN. 2000.
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