Download Leukocyte CoA level and N-acetytransferase activity as biomarkers

P003
Leukocyte CoA level and N-acetytransferase activity as
biomarkers of pantothenic acid allowances
Evgenij A. Moiseenok1, Sofia N. Omelyanchik2 and
Andrey G. Moiseenok2
1
Grodno State Medical University, Grodno, Belarus
2
Institute of Biochemistry of Biologically Active
Compounds, NAS of Belarus, Grodno, Belarus
Deficiencies of vitamin B5 and its coenzyme form (CoA) are
manifested in extreme situations, malnutrition, genetic disorders
such as “pantothenate kinase associated neurodegeneration”
(PKAN). More common is the syndrome of CASTOR (coenzyme
A sequestration, toxicity or redistribution) with abnormal mitochondrial metabolism due to sequestration or redistribution of CoA.
We were the first to apply in clinical research the level of CoA
and the activity of N-acetyltransferase (AT) in peripheral blood
leukocytes isolated by sedimentation in dextran. The level of CoA
was studied by a cyclic method with phosphotransacetylase and
was determined by accumulation of acetylhydroxamic acid. AT
activity was assayed by acetylation rate of p-aminobenzoic acid
(PABA) by a reaction with n-dimethylaminobenzaldehyde.
We examined 97 alcoholic patients, 44 patients with duodenal
ulcer and 22 apparently healthy individuals. The reference
values amounted to: CoA- 0.106±0.008 nmol x 10-6 leukocytes
and AT-11.31±nmol acetylated PABA min-1 mg-1 protein. The
alcoholic patients showed a more than 2-fold decrease of the
indices depending on the severity of alcohol abuse, which was
corrected by treatment with PaA preparations, but 7-to 14-day
treatment was not favorable for attaining the reference values.
The efficiency of the substances was in the following order:
pantethine>PaA-Ca>panthenol. The patients with duodenal ulcer
also demonstrated decreased indices of leukocyte AT and CoA,
which was corrected by peroral intake of PaA at a dose of
200-800 mg or 200 mg of pantethine.