Download RealTime ready Cell Lysis Kit

RealTime ready Cell Lysis Kit
Fast One-Step Cell Lysis for qRT-PCR
Substitute multi-step RNA purification with an easy singlestep cell lysis for direct use in cDNA synthesis followed by
high-performance real-time PCR.
 Increase convenience: Replace cumbersome multi-step
RNA purification protocols with a simple one-step cell
lysis procedure.
 Faster time to results: Lyse samples in just 5 minutes
at room temperature without further steps.
 Reduce intermediate steps: Perform the DNase
treatment for high quality results during the cDNA
synthesis incubation.
 Benefit from broad applicability: Scale-up from 3 to
30,000 cultured cells for real-time PCR applications in
96- to 1536-well formats with or without automation.
Convenience
RNA Preparation
cDNA Synthesis
Accelerated
Workflow
Real-Time PCR
Conventional
Workflow
30
60
90
120
150
Time-to-Result (min)
Figure 1: Time-savings and more convenience in two-step
qRT-PCR analysis of cell cultures*.
Reduce your hands-on time in half. Simplify your experimental workflow
by significantly reducing handling steps.
The Accelerated Workflow (single-step cell lysis, fast cDNA synthesis
combined with DNase treatment, real-time PCR) cuts your time in
half for gene expression analysis in comparison to the Conventional
Workflow (multi-step column-based RNA isolation, standard cDNA
synthesis, real-time PCR).
* Calculation of time for the preparation of 96 cell culture samples.
For life science research only.
Not for use in diagnostic procedure.
Experience Fast Gene Expression Analysis Directly from Cells!
High Pure RNA Isolation Kit (C q)
Figure 2: Real-time PCR results using the Accelerated Workflow
with the RealTime ready Cell Lysis Kit, and the Conventional
Workflow using the column-based High Pure RNA Isolation Kit.
Real-time PCR findings show accurate, reproducible results for both
RNA isolation techniques, resulting in high correlation of quantification
cycles (Cqs) for the 19 different reference genes tested.
Experimental setup: 30,000 HeLa cell equivalents/well were used for
RNA preparation. RNA was reverse transcribed using the Transcriptor
First Strand cDNA Synthesis Kit. Real-time PCR amplification was
performed on the LightCycler ® 480 Instrument (96-well format, 20 µl
reaction), using the LightCycler ® 480 Probes Master and RealTime
ready Human Reference Gene Panel.
RealTime ready Cell Lysis Kit (C q)
Retool your gene expression studies for high productivity!
Streamline your gene expression workflow using the new
RealTime ready Cell Lysis Kit and optimized cDNA synthesis
combined with DNase treatment.
Generate high-performance real-time PCR results using
reliable and sensitive RealTime ready assays and reagents
on LightCycler® Instruments.
qPCR results in just 75 minutes...
Cell
Cell Lysis
Lysis
cDNA Synthesis
DNase Treatment
Real-Time PCR
Ordering Information
Catalog
Number
Product
05 943 523 001
RealTime ready Cell
Lysis Kit
(500 x 40 µl reactions)
NEW!
Kit Contents:
RealTime ready Lysis Buffer, Protector
Catalog
Number
04 379 012 001
04 896 866 001
04 897 030 001
Product
Transcriptor First Strand cDNA
Synthesis Kit
1 Kit (50 x 20 µl reactions)
1 Kit (100 x 20 µl reactions)
1 Kit (200 x 20 µl reactions)
RNase Inhibitor, Thermolabile Nuclease
Catalog
Number
Product
04 707 494 001
04 887 301 001
5 x 1 ml (500 x 20 µl reactions)
LightCycler® 480 Probes Master
10 x 5 ml (5000 x 20 µl reactions)
LightCycler® 480 SYBR Green I Master
04 707 516 001
04 887 352 001
5 x 1 ml (500 x 20 µl reactions)
05 502 381 001
RealTime ready DNA Probes Master
(1,250 x 20 µl reactions)
10 x 5 ml (5000 x 20 µl reactions)
RealTime ready assays
Universal ProbeLibrary assays
05 015 278 001
LightCycler® 480 Instrument, 96-well
05 015 243 001
LightCycler® 480 Instrument, 384-well
05 334 276 001
LightCycler® 1536 Instrument
Learn more about Roche’s fast gene expression workflow at: www.gene-expression.roche.com
Published by
For life science research only.
Not for use in diagnostic procedure.
Roche Diagnostics GmbH
Roche Applied Science
Werk Penzberg
82372 Penzberg
Germany
© 2010 Roche Diagnostics GmbH
All rights reserved.
www.roche-applied-science.com
05836760001  0710
HIGH PURE, LIGHTCYCLER and REALTIME READY are trademarks of Roche.
Other brands or product names are trademarks of their respective holders.