Download Zakir Tnimov

Zakir Tnimov, PhD
Institute for Molecular Bioscience, University of Queensland, QBP Building 80, St Lucia 4072, Australia
Email: [email protected], Tel Lab: +61 7 334 62172, Mobile: +6146868456
Education and Work
Aug 2012 – present
Research officer at the group of Prof Kirill Alexandrov, Institute for Molecular Biosciences, University of
Queensland, Australia
Jan 2009 – Feb 2013
Ph.D. in Chemical Biology with Prof Kirill Alexandrov. Institute for Molecular Biosciences, University of
Queensland, Australia
Title “Novel approaches for understanding prenylation and Rho GTPase membrane targeting”
April 2012
Visiting scholar at the protein production group of Dr. Sunny Zhang, Seattle Biomedical institute, WA, USA
March 2012
Visiting scholar at the lab of Prof Arthur E. Johnson, A&M University, Texas, USA
Aug 2007 – Oct 2008
Research Scholar with Prof Kirill Alexandrov. Max-Planck Institute for Molecular Physiology, Dortmund,
Germany
Oct 2005 – Jul 2007
Master Science in Biology (with honors), M.V. Lomonosov Moscow State University, Moscow, Russia
Title: “Obtaining a hybrid Q-replicase containing T.thermophilus elongation factors”
With Prof Alexander Chetverin, Institute for Protein Research, Russian Academy Of Science, Puschino, Russia
Sep 2000 – Jun 2005
Specialist Diploma in Biochemistry (with honors), Udmurt State University, Izhevsk, Russia
Title: “Purification and characterization of elongation factors EF-Tu and EF-Ts of Thermus thermophilus”
With Prof Alexander Chetverin, Institute for Protein Research, Russian Academy Of Science, Puschino, Russia
Jun 2000
Abitur (University Entrance Qualification), Hi-School #52, Izhevsk, Russia
• Best performance in biology. I was awarded with first prizes on a number of Hi-school student research
conferences and specialized Olympiads from Ministry of Education, Russia.
Conferences and Meetings
8-12 Sep 2013 Protein Synthesis and Translational Control, EMBL, Heidelberg, Germany
Anticodon-specific replacement of tRNA isoacceptors by their orthogonal counterparts for site-selective
protein labeling in vitro.
26-30 Nov 2012 Cold Spring Harbor Asia, Synthetic Biology Conference, Suzhou, PRC
Towards effective multi-site protein labeling in cell-free protein synthesis systems.
Silver Poster Prize
22 Oct 2012 3rd P-CUBE User Meeting, EMBL Heidelberg, Germany
Towards effective multi-site protein labeling in cell-free protein synthesis systems.
6-10 Feb 2011 36th Lorne Conference on Protein Structure and Function, Lorne, Australia
The Mechanism of RhoGDI-mediated membrane targeting of RhoA. Second Poster Prize
9 -10 Nov 2011 Division of Chemistry and Structural Biology Symposium, Brisbane, Australia
Revealing the role of guanine nucleotide in RhoA recycling.
8-9 Jun 2010 Annual Scientific Conference, Institute of Protein Research, Pushchino, Russia
Chimeric Qbeta-replicase containing thermophilic EF-Ts
31 Jul-2 Aug 2009 East Coast Protein Meeting, Coffs Harbor, Australia
Thermodynamic and kinetic analysis of posttranslational modification and recycling of the RhoA
GTPase. Second Poster Prize
27 -30 Apr 2002 Actual medico-biological problems, Izhevsk State Medical Academy, Australia
Oral presentation: Immune profile of patients with breast cancer after radical cure treatment.
Schools and Workshops
13-16 Nov 2012 3rd Australian Biomolecular Small-Angle Scattering Workshop, Australian Synchrotron,
Clayton, VIC, Australia
23-24 Oct 2012 P-CUBE Workshop on Advanced Protein Engineering Techniques, EMBL Heidelberg,
Germany
6th Jul 2012
Presentation skills workshop, The University of Queensland, St Lucia, QLD, Australia
16th-10th Nov 2010
3rd Advanced Bio-Imaging Workshop, Sydney, Australia
6th-10th Jul 2009
Winter School in Mathematical and Computational Biology, St Lucia, Qld, Australia
3th-4th Mar 2009
Matlab Fundamentals, Brisbane, Qld, Australia
4th-15th Jun 2003
Summer School in Molecular Biology, Pushchino, Russia
Publications
[1]
Tnimov, Z., Guo, Z., Gambin, Y., Nguyen, T.-T., Wu, Y.-W., Abankwa, D., Stigter, A., Collins, B.,
Waldmann, H., Goody, R. S., and Alexandrov, K. (2012) Quantitative analysis of prenylated RhoA interaction
with its chaperone, RhoGDI. J. Biol. Chem.
[2]
Das, D., Tnimov, Z., Nguyen, U. T., Thimmaiah, G., Lo, H., Abankwa, D., Wu, Y., Goody, R. S.,
Waldmann, H., and Alexandrov, K. (2012) Flexible and general synthesis of functionalized phosphoisoprenoids
for the study of prenylation in vivo and in vitro. Chembiochem 13, 674-683
[3]
Raju R., Piggott A., Conte M., Tnimov Z., Alexandrov K., Capon R. Nocardiopsins: New FKBP12Binding Macrolide Polyketides from an Australian Marine-Derived Actinomycete, Nocardiopsis sp. 2010
Chemistry – A European Journal, 16(10):3194-3200
[4]
Rehman, A., Tnimov, Z., Hu S-H., Christie, M. P., Whitten, A. E., King, G., Jarrott, R., James, D. E.,
Alexandrov K., Collin, B.M., Martin, J. L.1 (2012) Un-tethered Syntaxin4 is obligatory for SNARE and
Munc18c complex assembly. In preparation
[5]
Tnimov Z., Norwood S., Guo Z., Abankwa D., Goody R.S. and Alexandrov K. Novel roles for RhoGDI
in RhoA prenylation and membrane targeting. In preparation
Technical Skills
Extensive Experience with:
• Kinetic and Thermodynamic characterization of protein:protein and protein:lipid interaction. Main techniques:
Fluorescence Spectroscopy, Fluorescence Anisotropy, Stopped-Flow, Isothermal Titration Calorimetry,
Fluorescence Correlation Spectroscopy and Fluorescence Cross-Correlation Spectroscopy and Microscale
Thermophoresis
• Non-linear least square regression of kinetic and equilibrium titration data using Dynafit, Scientist and Matlab
(or Octave)
• Chemo-enzymatic derivatization of recombinant proteins. Main techniques: expressed protein ligation, native
chemical ligation, protein trans-splicing, thiol and amine coupling
• Expression in E.coli and purification of recombinant proteins using FPLC: affinity interaction, hydrophobic,
ion-exchange and gel filtration chromatography
• Development of enzymatic assays for evaluation of steady-state enzymatic kinetic characteristics
• Specific amber codon- mediated protein labeling at two sites in in vitro translation system (Wheat Germ,
E.coli and Leishmania tarentolae extracts)
• Application and optimization of in vitro coupled transcription-translation systems for protein labeling using
puromycin, split-intein and native chemical ligation
• Large scale protein production in wheat germ extract
• MALDI, ESI-MS mass spectroscopy for routine determination of protein and small molecule molecular
weights
• Recombinant DNA methods including different in vitro mutagenesis and recombination strategies
• Validation and execution of real-time PCR protocols to quantify Baculovirus titer after transfection
• Optimization and execution of Western Blots to measure the expression of proteins and degree of their
labeling
• Processing of Small-Angle X-ray Scattering data and Multi-Angle Light Scattering for protein characterization
and structure determination
Familiar with:
• Cell culture techniques including basic microscopic imaging and FACS of transiently transfected mammalian
cells
• Amine coupling of small molecules and purification by reverse phase HPLC
Referees
Prof Kirill Alexandrov (Ph.D.
Supervisor)
E-mail:
[email protected]
Prof Roger S. Goody (Ph.D. CoSupervisor)
E-mail: [email protected]
Dr Brett Collins E-mail:
[email protected]
Institute for Molecular Biosciences, University of Queensland,
QBP Building 80, 4072 St Lucia, Brisbane, Australia Tel. +61 7
334 62017
Max Planck Institute of Molecular Physiology Dept. of Physical
Biochemistry
Otto-Hahn-Strasse 11 44227 Dortmund Germany
Institute for Molecular Biosciences, University of Queensland,
QBP Building 80, 4072 St Lucia, Brisbane, Australia Tel. +61 7
334 62043