Download Institutional/Department Administrative Activities

CURRICULUM VITAE
Wanguo Liu, Ph.D.
Personal
Department of Genetics &
Stanley S. Scott Cancer Center
Louisiana State University, Health Sciences Center
533 Bolivar Street, CSRB 416
New Orleans, LA 70112
Office Phone:
Lab Phone:
E-mail:
(504) 568-5143
(504) 568-2174
[email protected]
Current Position
Associate Professor,
Department of Genetics and Stanley S. Scott Cancer Center
Louisiana State University Health Sciences Center,
New Orleans, LA (2007 - )
Professional Positions & Academic Appointments
Senior Associate Consultant, Division of Experimental Pathology,
Mayo Clinic, Mayo Cancer Center, Rochester, MN (1999 - 07)
Associate Professor,
Department of Laboratory Medicine & Pathology,
Mayo Clinic College of Medicine, Rochester, MN (2006 - 07)
Assistant Professor,
Department of Laboratory Medicine & Pathology,
Mayo Clinic College of Medicine (1999 - 2006)
Prof. Associate in Research, Director of the Gene Identification Laboratory,
Mayo Clinic Cancer Center, Rochester, MN (1996 – 1999)
HHMI Research Associate,
Howard Hughes Medical Institute,
Stanford University Medical Center Stanford, CA (1993 -1996)
Research Assistant,
Department of Molecular Biology & Genetics,
Wayne State University, School of Medicine, Detroit, MI (1989 -1993)
Visiting Scientist,
Department of Biology,
University of Toledo, Toledo, OH (1987 -1989)
Lecturer,
Department of Biology,
Yunnan University, Kunming, China (1982 -1987).
1
Education
Postdoctoral fellow
HHMI, Stanford University Medical Center, Stanford, CA (1996)
Postdoctoral Research Fellow in Human Molecular Genetics
Sponsor: Dr. Uta Francke
Ph. D.
Wayne State University, School of Medicine, Detroit, MI (1993)
Molecular Biology and Human Genetics
Advisor: Dr. David I. Smith
M. S.
Yunnan University, Kunming, China (1982)
Cell Biology and Cytogenetics
B. S.
Yunnan Agricultural University, Kunming, China (1979)
Veterinary Science
Awards and Honors
Rumble Fellowship (1991-1993)
Institutional/Department Administrative Activities
LCRC Tissue Utilization Review Committee (TURC) (2008 - )
LSUHSC Stanley S. Scott Cancer Center Junior faculty mentoring committee (2007 - )
Mayo Clinic Safety Committee (2006 –2007)
Mayo Esophageal Adenocarcinoma and Barrett’s Esophagus Steering Committee (2000 –2007)
Research Committee of the Department of Laboratory Medicine and Pathology (1997 – 1999)
Journal Reviewer
CANCER RESEARCH
AMERICAN JOURNAL OF HUMAN GENETICS
ONCOGENE
HUMAN MUTATION
EXPERIMENTAL CELL RESEARCH
BRITISH JOURNAL OF CANCER
INTERNATIONAL JOURNAL OF CANCER
AMERICAN JOURNAL OF PATHOLOGY
GENES, CHROMOSOMES AND CANCER
Educational Activities
Teaching Activities
2008
“Special Topics in Cancer”
2007 Course INTER 123:
“Gene Expression Regulation”
2006 Tumor Biology Course: “Wnt signaling pathway in tumorigenesis”
2006 Tumor Biology Course: “From Bench-side to Bed-side-Special topics in the biology of cancer”
2
2004 Tumor Biology Course: “Wnt signaling pathway in colon cancer development”
2000 Genomics Course: “Biotechnology”
1982-86 Undergraduate courses: “Introduction of Biology,” “Cell Biology, Genetics,” “Principals and
Applications of Microscopes in Biology.”
Professional Membership and Societies
Member, American Society of Human Genetics
Member, American Association for Cancer Research
Research Interests
Genetics and Biological roles of the Wnt Signaling Defects in Tumorigenesis
DNA Damage-Signaling Defects in Prostate Cancer Susceptibility and tumorigenesis
Invited Speaker at National & International Meetings
2009
2006
2004
2002
2001
2000
2000
2000
2000
1998
1997
1997
1996
1995
1993
1992
1992
Wnt signaling meeting (Washington DC, USA)
The role of Dixin, an Axin2-binding protein, in colorectal cancer (Shanghai, China)
Axin2, a novel tumor suppressor in colorectal cancer (Beijing, China)
American Society of Human Genetics annual meeting (Baltimore, MD)
American Association of Cancer Research annual meeting (New Orleans, LA)
Chinese Academy of Medical Science & Peking Union Medical College (Beijing, China)
Beijing Institute for Cancer Research, Peking University (Beijing, China)
International Symposium on the State-of-the-Art in Genetic Analysis (Boston, MA)
American Society of Human Genetics annual meeting (Philadelphia, PA)
American Society of Human Genetics Annual Meeting (Denver, CO)
International symposium on the Separation of Protein, Peptides, Polynucleotides (Rockville, MD)
Symposium on Gene Expression & Mutation Analysis (San Francisco, CA)
International symposium on the Marfan syndrome (Davos, Switzerland)
American Society of Human Genetics Annual Meeting (Minneapolis, MN)
International chromosome-3 and Cancer Meeting (Groningen, Netherlands)
International Chromosome-3 Meeting (Tokyo, Japan)
American Society of Human Genetics Annual Meeting (San Francisco, CA)
Publications (Peer-reviewed & original articles in chronological order)
[1]
Liu W. and Zan R.G. Bands of chromosomes of four anuran amphibian and the relationship between the
C-banding, Ag-stained NORs and the 2nd constrictions. Acta Genetica Sinica 1984; 11:294-301.
[2]
Liu W. and Zan R.G. A special karyotype in the genus Rana: An investigation of the karyotype, C-banding
and Ag-stained NORs of Rana phrynoides Boulenger. Acta Genetica Sinica 1984; 11:61-7.
[3]
Zan R.G., Song Z., and Liu W. Studies of karyotypes of seven species of fishes in Barbinae, with a
discussion on identification of fish polyploids. Zoological Research 1984; 5:82-90.
[4]
Zan R.G., Song Z., and Liu W. Tetraploid-hexaploid relationship in Schizothoracinae. Acta Genetica
Sinica 1985; 12:137-142.
3
[5]
Smith DI, Liu W, Ginzinger D, Green P., Smith S., Wang N.D., Recchia F., Carolyn K., Drabkin H.,
Golembieski W. Localization of 616 human chromosome 3-specific cosmids during a somatic cell hybrid
deletion mapping panel. Genomics, 1991; 11:179-187.
[6]
Azuma T., Liu W., Wander Laan D.J., Bowcock A.M., and Taggart R.T. Human gastric cathepsin E gene.
J Biol Chem 1992; 267:1609-1614.
[7]
Liu W., Vance J., Smith D.I. cA479 (D3S719): A cosmid mapped telomeric of the Von Hippel Lindau
disease gene contains the D3S18 locus. Hum Mol Genet 1992; 1(3):201.
[8]
Liu W., Piechocki M., Shridhar V., Lyles G, Song Z., Nakamura Y., Drabkin H., Vance J., Smith D.I. The
isolation of a contig of overlapping yeast artificial chromosome (YAC) clones extending for 2.5 Mbs in
the vicinity of the von Hippel-Lindau disease gene. Hum Mol Genet 1993; 2(8):1177-1182.
[9]
Loeb D., Liu W., Smith D., Vance J. Dinucleotide repeat polymorphisms in the VHL region of human
chromosome 3p25. Hum Mol Genet 1993; 2(10):1746.
[10]
Swaroop A, Yang-Fang TL, Liu W, Gieser L, Barrow LL, Chen K, Agarwal N, MH, Smith D.I. Molecular
characterization of a novel human gene, SEC13R, related to the yeast secretory pathway gene SEC13, and
mapping to a conserved linkage group on human chromosome 3p24-p25 and mouse chromosome 6. Hum
Mol Genet 1994; 2(8):1281-86.
[11]
Loeb D., Liu W., Smith D., Vance J. Dinucleotide repeat polymorphisms in the VHL region. Hum Mol
Genet 1994; 3(3):520.
[12]
Francke U, Berg M, Tynan K, Brenn T, Liu W, Aoyama T, Gasner C, Miller C, Furthmayr H. A
Gly1127Ser mutation in EGF-like domain of the fibrillin-1 gene is a risk factor for ascending aortic
aneurysm and dissection. Am J Hum Gene. 1995; 56:1287-96.
[13]
Liu W., Qian C., Comeau K, Francke U. Mutant FBN1 monomers lacking EGF-like domains disrupt
microfibril assembly and cause severe Marfan syndrome. Hum Mol Genet 1996; 5:1581-1587.
[14]
Liu W., Faraco J, Qian C, Francke U. The gene for microfibril-associated protein-1 (MFAP1) is located
several megabases proximal to FBN1 and is not mutated in Marfan syndrome. Hum Genet 1997; 99:578584.
[15]
Schrijver I., Liu W., Francke U. The pathogenicity of the Por1148Ala substitution in the FBN1 gene:
Causing or predisposing to Marfan syndrome and aortic disease, or clinically innocent? Hum Genet
1997; 99:607-611.
[16]
Liu W., Qian C., Francke U. Silent mutation causes exon skipping of FBN1 gene in Marfan syndrome.
Nat Genet 1997; 16:328-329.
[17]
Liu W., James C.D, Frederick L., Alderete, B.E. and Jenkins, R.B. PTEN/MMAC1 mutations and EGFR
amplification in Glioblastomas. Cancer Res. 1997; 57:5254-5257.
[18]
Collod-Beroud G., Beroud C., Ades L., Black C., Boxer M., Brock DJH., Holman KJ., De paepe A.,
Francke U., Grau U., Hayward C., Klein HG., Liu W., Nuytinck L., Peltonen L., Alvarez Perez AB.,
Rantamaki T., Juniwn C., Boileau C. Marfan Database I third edition): new mutations and new routines for
the software. Nucleic Acids Res. 1998; 26:231-236.
[19]
Jenkins R.B., Qian J., Lee H.K., Huang H., Hirasawa K., Bostwick D.G., Proffitt J., Wilber K., Lieber
M.M., Liu W. and Smith D.I. A molecular cytogenetic analysis of 7q31 in prostate cancer. Cancer Res.
1998; 58:759-766.
4
[20]
Liu, W., Smith, D. I., Thibodeau, S., and James, C. D. Denaturing high performance liquid
chromatography (DHPLC) used in the detection of germline and somatic mutations. Nucleic Acid Res.
1998; 26:1396-1400.
[21]
Mai, M., Yokomizo, A., Qian, C., Yang, P., Tindall, D. J., Smith, D. I. and Liu W. Activation of p73 silent
allele in lung cancer. Cancer Res. 1998; 58:2347-2349.
[22]
Wang, X-Y., Smith, D.I., Liu, W. and James, C. D. GBAS, a noevl gene encoding a protein with
tyrosinephosphorylation sites and a transmembrane domain, is co-amplified with EGFR. Genomics 1998;
49:448-451.
[23]
Yokomizo A., Tindall D J., Jenkins R B., Smith D I., and Liu W. Mutation analysis of putative tumor
suppressor, PTEN/MMCA1 gene in human ovarian cancer. Inter J Oncol 1998; 13:101-105.
[24]
Yokomizo A., Tindall D.J., Drabkin H., Gemmill R., Franklin W., Yang P., Sugio K., Smith D.I. and Liu
W. PTEN/MMAC1 mutations identified in small ell, but not in non-small cell lung cancers. Oncogene
1998; 17: 475-479.
[25]
Mai M., Huang, H., Reed, C., Qian, C., Smith, S., Alderete, B., Jenkins, R., Smith, D. I. and Liu W.
Genomic organization and mutation analysis of p73 in oligodendrogliomas with chromosome 1p-arm
deletions. Genomics 1998; 51: 359-363.
[26]
Liu W., Oefner P., Qian C., Odom R. and Francke U. Denaturing HPLC identified novel FBN1 mutations,
polymorphisms and sequence variants in Marfan syndrome patients. Genetic Testing 1998; 1:237-242.
[27]
Mai, M., Qian, C., Yokomizo, A., Tindall, D. J., Bostwick, D., Polychronakos, C., Smith, D. I. and Liu W.
Loss of imprinting and allele switching expression of p73 in renal cell carcinoma. Oncogene 1998;
1:1739-41.
[28]
Zhang J., Nelson M., Wang L., Liu W., Qian C., Shridhar V., Urritia R., and Smith DI. Identification and
chromosomal localization CTNNAL1, a novel homolog to -catein. Genomics 1998; 54:149-54.
[29]
Mai M., Qian C., Yokomizo A., Smith DI., and Liu W. Cloing of Human Conductin (AXIN2), a Gene
Mapping to Chromosome 17q23-24. Genomics 1999; 55:341-344.
[30]
Wang L., Darling J., Zhang J.S., Huang H., Liu W. and Smith D.I. Allele-specific late replication and
fragility of the most active common fragile site, FRA3B. Hum Mol Genet 1999, 8:431-437.
[31]
Yokomizo A., Mai M., Tindall DJ., Cheng L., Bostwick DG., Naito S., Smith DI., and Liu W.
Overexpression of the wild type p73 gene in invasive bladder cancer. Oncogene 1999, 18:1629-33.
[32]
Yokomizo A., Mai M., Bostwick DG., Tindall DJ., Qian J., Cheng L., Smith DI., and Liu W. Mutation and
Expression Analysis of the p73 Gene in Prostate Cancer. Prostate 1999, 39:94-100.
[33]
Schrijver I., Liu W (co-first author)., Brenn T., Furthmayr H and Francke U. Cysteine Substitutions in
EGF-like Domains of Fibrillin-1: Distinct Effects on Biochemical and Clinical Phenotypes.
Am J Hum Genet 1999, 65:1007-1020.
[34]
Schwartz DI, Lindor NM, Walsh-Vockley C, Roche PC, Mai M, Smith DI, Liu W, and Couch FJ. P73
mutations are not associated with sporadic and familial breast cancer. Breast Cancer Res Tr. 1999,
58:25-29.
5
[35]
Wang L., Darling J., Zhang J.S., Liu W., Qian J., Bostwick D., Hartmann L., Jenkins R. and Smith D.I.
Loss of expression of the DRR-1 gene at chromosomal band 3p21.1 in renal cell carcinoma. Genes,
Chromosomes & Cancer 2000, 27:1-10.
[36]
Seelan RS, Qian C, Yokomizo A, Bostwick DG, Smith DI, and Liu W. Human Acid Ceramidase is
overexpressed but not mutated in Prostate Cancer. Genes, Chromosomes, Cancer 2000, 29:137-46.
[37]
Liu W, Mai M, Yokomizo A, Qian C, Tindall DJ, Smith DI, and Thibodeau SN. Differential expression
and allelotyping of the p73 gene in neuroblastoma. Inter J Oncol 2000, 16:181-5.
[38]
McIver B, Grebe SK, Wang L, Hay ID, Yokomizo A, Liu W, Goellner JR, Grant CS, Smith DI, Eberhardt
NL. FHIT and TSG101 in thyroid tumours: aberrant transcripts reflect rare abnormal RNA processing
events of uncertain pathogenetic or clinical significance. Clin Endocrinol 2000, 52:749-57.
[39]
Vockley J., Anderson BD., Willard J., Seelan S., Smith DI., and Liu W. An Unusually High Frequency of
Abnormal Splicing of IVD RNA in Isovaleric Acidemia, Including Exon Skipping Caused by Missense
Mutation in the IVD Gene. Am J Hum Genet 2000, 66:356-367.
[40]
Krishnadath KK, Wang KK, Taniguchi K, Sebo TJ, Buttar NS, Anderson MA, Lutzke LS, Liu W.
Persistent genetic abnormalities in Barrett's esophagus after photodynamic therapy. Gastroenterology
2000, 119(3):624-30.
[41]
Liu W, Dong X, Mai M, Seelan RS, Taniguchi K, krishnadath KK, Halling KC, Cunningham JM, Qian C,
Christensen E, Roche PC, Smith DI and Thibodeau SN. Mutations in AXIN2 cause colorectal cancer with
defective mismatch repair by activating β-catenin-Tcf signaling. Nat Genet 2000, 26:146-147.
[42]
Irwin M, Marin MC, Phillips AC, Seelan RS, Smith DI, Liu W, Vousden KH and Kaelin WG. Role for the
p53 homolog p73 in E2F1-induced Apoptosis. Nature 2000, 407:645-648.
[43]
Dong X, Seelan RS, Qian C, and Liu W. Genomic Structure, chromosome mapping and expression
analysis of the human AXIN2 gene. Cytogenet Cell Genet. 2001, 93:26-8.
[44]
Lindor NM, Dechet CB, Greene MH, Jenkins RB, Zincke MT, Weaver AL, Wilson M, Zincke H, Liu W.
Papillary renal cell carcinoma: analysis of germline mutations in the MET proto-oncogene in a clinicbased population. Genet Test. 2001, 5(2):101-6.
[45]
Liu W, Schrijver I, Brenn T, Furthmayr H, Francke U. Multi-exon deletions of the FBN1 gene in Marfan
syndrome. BMC Med Genet 2001; 2(1):11.
[46]
Yang P, Yokomizo A, Tazelaar HD, Marks RS, Lesnick TG, Miller DL, Sloan JA, Edell ES, Meyer RL,
Jett J, and Liu W. Genetic determinants of lung cancer short-term survival: the role of glutathione-related
genes. Lung Cancer 2002, 35(3):221-9.
[47]
Lingle WL, Barrett SL, Negron VC, D’Assoro AB, Boeneman K, Liu W, Whitehead CM, Reynolds C, and
Salisbury JL. Centrosome Amplification Drives Chromosomal Instability in Breast Tumor Development.
Proc Natl Acad Sci USA 2002, 99(4): 1978-83.
[48]
Seelan RS, Irwin M, van der Stoop P, Qian C, Kaelin WG, and Liu W. The human p73 promoter:
Characterization and identification of functional E2F binding sites. Neoplasia, 2002, 4(3):195-203.
[49]
Taniguchi K, Yang P, Jett J, Bass E, meyer R, Wang Y, Deschamps C, and Liu W. The role of neutrophil
elastase in lung cancer development: evidence from molecular biology and clinical epidemiology. Clin
Cancer Res. 2002, 8(4):1115-20.
6
[50]
Taniguchi K, Dong X, Qian C, Aderca, IN, Murphy LM, Nagorney DM, Burgart LJ, Roche PC, Smith DI,
Ross JA, Roberts LR, and Liu W. Mutation spectrum of beta-catenin, AXIN1, and AXIN2 in
Hepatocellular Carcinomas and Hepatoblastomas. Oncogene, 2002, 21(31):4863-71.
[51]
Schrijver I., Liu W (co-first author), Odom R., Brenn T., furthmayr H., and Francke U. Premature
Termination Mutations in FBN1: Distinct Effects on Differential Allelic Expression, Protein and Clinical
Phenotypes. Am J Hum Genet 2002, 71(2):223-37.
[52]
Elkins DA, Yokomizo A, Thibodeau SN, Schaid DJ, Cunningham JM, Marks A, Christensen E,
McDonnell SK, Slager S, Peterson BJ, Jacobsen SJ, Cerhan JR, Blute ML, Tindall DJ, and Liu W.
Luteinizing Hormone  Polymorphism and Risk of Familial and Sporadic Prostate Cancer. Prostate, 2003,
56(1):30-6.
[53]
Dong X, Wang L, Taniguchi K, Wang X, Cunningham JM, McDonnell SK, Qian C, Marks AF, Slager SL,
Peterson BJ, Smith DI, Cheville JC, Blute ML, Jacobsen SJ, Schaid DJ, Tindall DJ, Thibodeau SN & Liu W.
Mutations in CHEK2 Associated with Prostate Cancer Risk. Am J Hum Genet 2003, 72:270-280.
[54]
Pan K, Liu W, Lu YY, Zhang L, Li ZP, Lu WL, Thibodeau SN, and You WC. High throughput detection
of microsatellite instability by denaturing high-performance liquid chromatography. Hum Mut 2003,
22:388-394.
[55]
Debes JD, Yokomizo A, McDonnell SK, Hebbring SJ, Christensen GB, Cunningham JM, Jacobsen SJ,
Tindall DJ, Liu W, Schaid DJ, Thibodeau SN. Gluthatione-S-transferase P1 polymorphism I105V in
familial and sporadic prostate cancer. Cancer Genet Cytogenet. 2004, 155(1):82-6.
[56]
Chien J, Staub J, Avula R, Zhang H, Liu W, Hartmann LC, Kaufmann SH, Smith DI, Shridhar V.
Epigenetic silencing of TCEAL7 (Bex4) in ovarian cancer. Oncogene. 2005, 24(32):5082-5100.
[57]
Chen Y, Zhou G, Yu M, He Y, Tang W, Lai J, He J, Tan D, and Liu W. Cloning and functional analysis of
human mTERFL encoding a novel mitochondrial transcription termination factor-like protein. Biochem
Biophys Res Commun. 2005, 337(4):1112-8.
[58]
Koinuma K, Yamashita Y, Liu W, Hatanaka H, Kurashina K, Wada T, Takada S, Kaneda R, Choi YL,
Fujiwara SI, Miyakura Y, Nagai H, Mano H. Epigenetic silencing of AXIN2 in colorectal carcinoma with
microsatellite instability. Oncogene. 2005, 10;1-8.
[59]
Wu X, Dong X, Chen J, and Liu W. Characterization of Chk2 mutations in prostate cancer. Hum Mut
2006, 27(8):742-747.
[60]
Wang X, Zheng L, Zeng Z, Zhou G, Chien J, Qian C, Vasmas G, Shridhar V, Chen L, and Liu W.
DIXDC1 isoform, l-DIXDC1, is a novel filamentous actin-binding protein. Biochem Biophys Res
Commun. 2006, 347(1):22-30.
[61]
Zheng L, Wang F, Neumann RM, Chville JC, Tindall DJ, and Liu W. Mutually exclusive mutations of
CHEK2 and TP53 implicated in primary prostate tumors and cancer cell lines. Hum Mut 2006,
27(10):1062-63.
[62]
Wang X, Taniguchi K, Seelan RS, Wang L, McDonnell SK, Qian C, Pan K, Lu Y, Shridhar V, Couch FJ,
Tindall DJ, Cooney KA, Isaacs WB, Jacobsen SJ, Schaid DJ, Thibodeau SN, and Liu W. Germline
p53AIP1 Mutations Disrupting DNA Damage-induced Apoptosis are Associated with Sporadic Prostate
Cancer. Cancer Res 2006, 66(21):10302-10307.
[63]
Lee H, Kim D, Dan HC, Wu El, Gritsko TM, Cao C, Nicosia SV, Golemis EA, Liu W, Coppola D, Drem
SS, Testa JR, and Cheng JQ. Identification and characterization of putative tumor suppressor NGB, a
GTP-binding protein that interacts with the neurofibromatosis 2 protein. Mol Cell Biol. 2007, 27:2103-19.
7
[64]
Wang X, Szabo C, Qian C, Amadio PG, Thibodeau SN, Cerhan JR, Petersen GM, Liu W, Couch FJ.
Mutational analysis of thirty-two double-strand DNA break repair genes in breast and pancreatic cancers.
Cancer Res. 2008 68(4):971-5.
[65]
Wang L, Oberg AL, Asmann YW, Sicotte H, McDonnell SK, Riska SM, Liu W, Steer CJ, Subramanian S,
Cunningham JM, Cerhan JR, Thibodeau SN. Genome-wide Transcriptional Profiling Reveals MicroRNAcorrelated Genes and Biological Processes in Human Lymphoblastoid Cell Lines. PLoS ONE 2009,
11:4(6)e5878.
8