Survey
* Your assessment is very important for improving the work of artificial intelligence, which forms the content of this project
Download Powerpoint - Blood Journal
Document related concepts
no text concepts found
Transcript
Ectopic expression of TAL-1 protein in Ly-6E.1-htal1transgenic mice induces defects in B- and T-lymphoid differentiation by Nicolas Goardon, Annette Schuh, Iman Hajar, Xiaoqian Ma, Hélène Jouault, Elaine Dzierzak, Paul-Henri Roméo, and Leı̈ la Maouche-Chrétien Blood Volume 100(2):491-500 July 15, 2002 ©2002 by American Society of Hematology Ly-6E.1 htal-1 and Ly-6E.1 Δbhtal-1 vectors and transgene expression.(A) The Ly-6E.1 vector contains the 14-Kb Ly-6E.1genomic sequence, including promoter and 3′ sequence of theLy-6E.1 gene. Nicolas Goardon et al. Blood 2002;100:491-500 ©2002 by American Society of Hematology Morphology of peripheral CD11b+ cells.Microscopic appearance of representative cytospins revealed by May-Grünwald-Giemsa (original magnification, × 600) showing transgenic (Tg) and wild-type (Wt) peripheral CD11b+myeloid cells (monocyte, mature granulocyte,... Nicolas Goardon et al. Blood 2002;100:491-500 ©2002 by American Society of Hematology Consequences of hTAL-1 expression on thymus cellularity.(A) hTAL-1 protein expression in the thymus. Nicolas Goardon et al. Blood 2002;100:491-500 ©2002 by American Society of Hematology Defect in early B lymphopoiesis of transgenic mice.(A) Phenotypic analysis of BM B cells stained with anti-B220–FITC and anti-CD19–PE monoclonal antibodies. Nicolas Goardon et al. Blood 2002;100:491-500 ©2002 by American Society of Hematology Htal-1 transgenic mice show a reduction in splenocytes, disorganization of the germinal center, and a defect in antigen-dependent B-cell maturation.(A) Disorganization of germinal centers (GC) in the spleen of transgenic mice. Nicolas Goardon et al. Blood 2002;100:491-500 ©2002 by American Society of Hematology The reduction in the B-cell compartment and the persistence of the Sca-1 antigen on myeloid cells because of ectopic TAL-1 expression are cell autonomous phenomena.(A) Peripheral blood cells of NOD/SCID mice transplanted withhtal-1 transgenic (Tg) or wild-t... Nicolas Goardon et al. Blood 2002;100:491-500 ©2002 by American Society of Hematology
Related documents