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This literature thesis describes affinity chromatographic techniques and applications. All the different types of affinity chromatography, as well as their recent applications and developments are presented. More specifically, types of affinity chromatography such as lectin, boronate, immunoaffinity, immobilized metal-ion, protein A, G and L, analytical, dye-ligand, bioaffinity chromatography and more, are reported and their advantages and drawbacks are analyzed.