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Product Datasheet Recombinant Rabbit Anti-NT-proBNP/N-terminus Antibody Cat. No: DA-2004 PRODUCT INFOMATION Product Name Recombinant Rabbit Monoclonal Antibody Specific for NT-proBNP/N-terminus Gene ID 4879 Target Human NT-proBNP/N-terminus UniProt ID P16860 Immunogen Synthetic peptides derived from human NT-proBNP/N-terminus Species Reactivity Human Clone S2H8 Type Expression Host Recombinant Monoclonal Rabbit IgG / light chain kappa HEK293F Purification Protein A purified Affinity 0.03 x 10 M Modification None -9 Applications WB, dilution (1:1000 – 1: 5000) and sandwich ELISA, pairs with clone S3A10 Capture antibody: clone S3A10 (Cat#DA-2003) Detection antibody: clone S2H8 (Cat#DA-2004) Optimal dilutions /concentrations should be determined by the end user Format PBS, pH 7.2, 0.02% NaN3 Storage Store at 4°C for up to 6 months. Store at -20°C long term. DATA (Western blot and Sandwich ELISA) Antibody: (DA-2004) at 1/1000 dilution Sample: 30 ng recombinant pro-BNP NTproBNP Secondary HRP conjugated anti-Rabbit Fc at 1/50000 dilution Predicted band size: 9 kDa Observed band size: 9 kDa Gel concentration: 4-20% AvantGen, Inc., 10151 Pacific Mesa Blvd., Ste 103, San Diego, CA 92121; Tel: 858-768-8107; Fax: 858-587-6692; www.avantgen.com "FOR RESEARCH AND DIAGNOSTIC USE ONLY AND ARE NOT INTENDED FOR THERAPEUTIC USE"