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Download 1409-1414 August 15, 2000
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Platelet endothelial cell adhesion molecule-1 (PECAM-1) is a target glycoprotein in drug-induced thrombocytopenia by Hartmut Kroll, Qi-Hong Sun, and Sentot Santoso Blood Volume 96(4):1409-1414 August 15, 2000 ©2000 by American Society of Hematology Carbimazole-dependent antibodies in enzyme immunoassay.Binding of carbimazole-dependent antibodies in sera from 5 patients in the presence of the drug (1 mg/mL, solid bars) and without drug (open bars). Hartmut Kroll et al. Blood 2000;96:1409-1414 ©2000 by American Society of Hematology Carbimazole-dependent antibodies in a glycoprotein-specific enzyme immunoassay (MAIPA).Reactivities of carbimazole-dependent antibodies against PECAM-1 immobilized by mabs in the presence of the drug. Hartmut Kroll et al. Blood 2000;96:1409-1414 ©2000 by American Society of Hematology Analysis of carbimazole-dependent antibodies by flow cytometry.Fixed platelets were incubated with patient sera in the absence of the drug (A), in the presence of the drug (B), and in the presence of the drug and soluble r-PECAM-1 (C). Hartmut Kroll et al. Blood 2000;96:1409-1414 ©2000 by American Society of Hematology Immunoprecipitation analysis of carbimazole-dependent antibodies.Platelets (A) and endothelial cells (EC) (B) were surface-labeled with biotin and lysed. Hartmut Kroll et al. Blood 2000;96:1409-1414 ©2000 by American Society of Hematology Reactivity of carbimazole-dependent antibodies with platelets and endothelial cells.Carbimazoledependent antibodies were analyzed in MAIPA assay with endothelial cells (EC) or platelets (Plts) either in the presence (solid bars) or in the absence (open bar... Hartmut Kroll et al. Blood 2000;96:1409-1414 ©2000 by American Society of Hematology Flow cytometric analysis of platelets with different PECAM-1 isoforms.Fixed platelets derived from individuals carrying PECAM-1 Leu125 (A) or Val125 (B) isoforms were analyzed with patient sera in the presence or in the absence of the drug (see Figure 3). Hartmut Kroll et al. Blood 2000;96:1409-1414 ©2000 by American Society of Hematology Immunoprecipitation analysis of carbimazole-dependent antibodies with PECAM-1 mutants.Untransfected L-cells and L-cells expressing the entire PECAM-1 or lacking the first (Δ1) or the second (Δ2) extracellular immunoglobulin homology domain were surface labe... Hartmut Kroll et al. Blood 2000;96:1409-1414 ©2000 by American Society of Hematology
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