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Antiadhesive function of 130-kd glycoform of CD43 expressed in CD4 T-lymphocyte clones and transfectant cell lines by Mami Fukuoka, Kenji Fukudome, Yoshio Yamashita, Miyoko Tokushima, Kensuke Miyake, and Masao Kimoto Blood Volume 96(13):4267-4275 December 15, 2000 ©2000 by American Society of Hematology Flow cytometric analysis of MFT3 antigen expression in T-cell clones and in cells from various lymphoid tissues.Autoreactive T-cell clones KGU139 (A) and KGU140 (B) derived from B/WF1 mice were stained with MFT3 monoclonal antibody (mAb; IgG2a) followed by ... Mami Fukuoka et al. Blood 2000;96:4267-4275 ©2000 by American Society of Hematology Biochemical characterization of MFT3 antigen as the 130-kd CD43 glycoform.(A) Lysates of bone marrow cells from BALB/c mice were subjected to sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE; 6% polyacrylamide gels), and Western blotting ... Mami Fukuoka et al. Blood 2000;96:4267-4275 ©2000 by American Society of Hematology MFT3 mAb reacted with CD43 gene products.293 cells were stably transfected with CD43 complementary DNA (cDNA), stained with MFT3 (A) or S7 (B) mAb followed by FITC-MAR18.5, and analyzed on a FACScan. Mami Fukuoka et al. Blood 2000;96:4267-4275 ©2000 by American Society of Hematology C2GnT transcripts were detected in MFT3-positive but not MFT3-negative cells on analysis with RT-PCR.(A) Total RNA from MFT3 antigen–positive (KGU139) and MFT3 antigen–negative (KGU140) T-cell clones was subjected to RT and amplified by PCR using C2GnT-spec... Mami Fukuoka et al. Blood 2000;96:4267-4275 ©2000 by American Society of Hematology MFT3-negative T-cell clones showed increased homocytic aggregation compared with MFT3positive T-cell clones.MFT3-negative T-cell clones KGU140 (A) and KSB2 (B) and MFT3-positive T-cell clones KGU139 (C) and KGX114 (D) were cultured in the presence or absen... Mami Fukuoka et al. Blood 2000;96:4267-4275 ©2000 by American Society of Hematology Antiadhesive function of the 130-kd CD43 glycoform on T cells.T-cell clones (A) from B/WF1 mice positive for MFT3 antigen (KGU139, ■) and negative for MFT3 antigen (KGU140, ░) and Tcell lines (B) from BALB/c mice positive for MFT3 antigen (SA-3, ■) and neg... Mami Fukuoka et al. Blood 2000;96:4267-4275 ©2000 by American Society of Hematology Induction by C2GnT of expression of the 130-kd CD43 glycoform in CD43 transfectant cells.BALB/3T3 (3T3) fibroblastic cell lines (A,B) were transfected with CD43 cDNA (C,D) or cotransfected with CD43 and C2GnT cDNA (E,F). Mami Fukuoka et al. Blood 2000;96:4267-4275 ©2000 by American Society of Hematology Antiadhesive function was carried out by the 130-kd glycoform of CD43 but not the 115-kd glycoform.(A,B) BALB/3T3 cell lines stably transfected with no CD43 (3T3), CD43 (3T3-CD43), CD43 plus C2GnT (3T3-CD43-C2GnT), or C2GnT (3T3-C2GnT) cDNA were cultured in... Mami Fukuoka et al. Blood 2000;96:4267-4275 ©2000 by American Society of Hematology
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