Download Recommended Search parameters for search startline LC

Mascot MS/MS Ions Search:
Recommended Search parameters for search LC-ESI-MS/MS data:
Database: NCBInr or SwissProt
Taxonomy: Proteobacteria or Other Proteobacteria, or Helicobacter, if accessible
Enzyme: Trypsin
Allow up to: 1 missed cleavage
Fixed modifications: none
Variable modifications: N-Acetyl (Protein), Oxidation (M), Propionamide (C), Pyro-Glu (Nterm Q)
Protein mass: none
ICAT: none
Peptide tolerance: 0.4 Da,
MS/MS tol. +/-: 0.4 Da
Peptide charge: 1+,2+,3+
Monoisotopic
Data file: paste the data into a text program (e.g. textpad), store it and transfer it into
MASCOT by Browse
Data format: Mascot generic
Precursor: none
Instrument: ESI-TRAP
Overview: none
Report top: 40 hits
Start search
Recommended Search parameters for search 2-DE-MALDI-MS and MS/MS data:
Database: NCBInr or SwissProt
Taxonomy: Proteobacteria or Other Proteobacteria, or Helicobacter, if accessible
Enzyme: Trypsin
Allow up to: 1 missed cleavage
Fixed modifications: none
Variable modifications: N-Acetyl (Protein), Oxidation (M), Propionamide (C), Pyro-Glu (Nterm Q)
Protein mass: none
ICAT: none
Peptide tolerance: 30 ppm,
MS/MS tol. +/-: 0.3 Da
Peptide charge: 1+
Monoisotopic
Data file: paste the data into a text program (e.g. textpad), store it and transfer it into
MASCOT by Browse
Data format: Mascot generic
Precursor: none
Instrument: MALDI-TOF-TOF
Overview: none
Report top: 40 hits
Start search