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Fig. 1. Proper paraformaldehyde fixation preserves the architecture of the actin cytoskeleton and is compatible with high-quality SMLM. HeLa (A-C) and Cos-7 (D-F) cells fixed with paraformaldehyde (PFA) dissolved in PBS (A and D), PFA in PEM buffer (B and E) or glutaraldehyde (GA) in cytoskeleton buffer (C and F). Daniela Leyton-Puig et al. Biology Open 2016;5:1001-1009 © 2016. Published by The Company of Biologists Ltd