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Download Chapter 15 - Assays for Cell Viability, Proliferation and Function
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15 Chapter 15 Assays for Cell Viability, Proliferation and Function Section 15.1 — Overview of Probes for Cell Viability, Cell Proliferation and Live-Cell Function . . . . . 603 Section 15.2 — Viability and Cytotoxicity Assay Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 604 Viability/Cytotoxicity Assays Using Esterase Substrates ............................................................................................................................ Calcein AM, Dihydrocalcein AM and Calcein Blue AM ........................................................................................................................ BCECF AM .......................................................................................................................................................................................... Fluorescein Diacetate ......................................................................................................................................................................... Carboxyfluorescein Diacetate and Its Derivatives ............................................................................................................................... Sulfofluorescein Diacetate .................................................................................................................................................................. CellTracker Green CMFDA .................................................................................................................................................................. Pentafluorobenzoyl Aminofluorescein Diacetate ................................................................................................................................ Chloromethyl SNARF-1 Acetate ......................................................................................................................................................... Carboxynaphthofluorescein Diacetate ................................................................................................................................................ Viability/Cytotoxicity and Gram Stain Assays Using Nucleic Acid Stains ................................................................................................... SYTOX Green, SYTOX Orange and SYTOX Blue Nucleic Acid Stains .................................................................................................. Dimeric and Monomeric Cyanine Dyes .............................................................................................................................................. Ethidium and Propidium Dyes ............................................................................................................................................................ Ethidium Monoazide .......................................................................................................................................................................... Hexidium Iodide: A Fluorescent Gram Stain ....................................................................................................................................... SYTO Dyes ......................................................................................................................................................................................... FUN 1 and FUN 2: Unique Stains for Assessing Viability of Yeast and Fungi ...................................................................................... 7-Aminoactinomycin D ...................................................................................................................................................................... Viability/Cytotoxicity Assays That Measure Oxidation or Reduction .......................................................................................................... High-Purity Resazurin ........................................................................................................................................................................ Dodecyl Resazurin: A Superior Probe for Cell Metabolic Studies ....................................................................................................... Dihydrorhodamines and Dihydrofluoresceins .................................................................................................................................... RedoxSensor Red CC-1 Stain ............................................................................................................................................................ Tetrazolium Salts ................................................................................................................................................................................ Other Viability/Cytotoxicity Assay Methods ............................................................................................................................................... Potentiometric Dyes ........................................................................................................................................................................... Acidotropic Stains .............................................................................................................................................................................. Fluorescent Glucose Analogs ............................................................................................................................................................. Fluorescent Antibiotics and Related Probes ............................................................................................................................................... Fluorescent Polymyxins ..................................................................................................................................................................... Fluorescent Penicillin Analogs ........................................................................................................................................................... BODIPY FL Vancomycin ..................................................................................................................................................................... Antimalarial Agents ............................................................................................................................................................................ Data Table .................................................................................................................................................................................................. Product List ............................................................................................................................................................................................... 604 604 605 606 606 606 606 607 607 607 607 607 608 609 610 610 610 611 611 611 611 611 611 612 612 612 613 613 613 613 613 614 614 614 615 617 Section 15.3 — Viability and Cytotoxicity Assay Kits for Diverse Cell Types . . . . . . . . . . . . . . . . . . 618 Viability Assay Kits for Animal Cells .......................................................................................................................................................... LIVE/DEAD Viability/Cytotoxicity Kit for Animal Cells ......................................................................................................................... LIVE/DEAD Reduced Biohazard Cell Viability Kit #1 ........................................................................................................................... LIVE/DEAD Reduced Biohazard Cell Viability Kits #2, #3 and #4 ........................................................................................................ 618 618 619 620 599 LIVE/DEAD Cell-Mediated Cytotoxicity Kit .......................................................................................................................................... LIVE/DEAD Sperm Viability Kit ........................................................................................................................................................... Vybrant Tumor Necrosis Factor (TNF) Assay Kit ................................................................................................................................ Vybrant Cell Metabolic Assay Kit ........................................................................................................................................................ Vybrant Cytotoxicity Assay Kit ........................................................................................................................................................... Viability Assay Kits for Yeast and Bacteria ................................................................................................................................................. LIVE/DEAD Yeast Viability Kit ............................................................................................................................................................. LIVE/DEAD Bac Light Bacterial Viability Kits ....................................................................................................................................... LIVE Bac Light Bacterial Gram Stain Kit .............................................................................................................................................. ViaGram Red+ Bacterial Gram Stain and Viability Kit .......................................................................................................................... Wheat Germ Agglutinin Sampler Kit ................................................................................................................................................... ATP Determination ..................................................................................................................................................................................... Product List ............................................................................................................................................................................................... 621 621 622 624 624 625 625 626 628 629 630 630 631 Section 15.4 — Assays for Cell Enumeration, Cell Proliferation and Cell Cycle . . . . . . . . . . . . . . . . 631 Cell Enumeration and Cell Proliferation Assays for Animal Cells ............................................................................................................... Proliferation Assays Using Bromodeoxyuridine Incorporation ........................................................................................................... Proliferation Assays Using ChromaTide Nucleotides .......................................................................................................................... ABSOLUTE-S SBIP Cell Proliferation Assay Kit .................................................................................................................................. Proliferation Assay Using the Succinimidyl Ester of Carboxyfluorescein Diacetate and Related Probes ............................................ Vybrant DiI Cell-Labeling Solution ..................................................................................................................................................... CyQUANT Cell Proliferation Assay Kit ................................................................................................................................................ Vybrant MTT Cell Proliferation Assay Kit ............................................................................................................................................ FluoReporter Blue Fluorometric Nucleic Acid Assay Kit ..................................................................................................................... Detection and Enumeration Assays for Microorganisms and Viruses ........................................................................................................ Detecting Bacteria, Yeast and Plankton Using Nucleic Acid Stains ..................................................................................................... Detecting Viruses Using Nucleic Acid Stains ...................................................................................................................................... Bacteria Counting Kit ......................................................................................................................................................................... Cell Culture Contamination Detection Kit ........................................................................................................................................... MycoFluor Mycoplasma Detection Kit ................................................................................................................................................ Nucleic Acid Probes for Cell-Cycle Analysis .............................................................................................................................................. SYTOX Green and SYTO Nucleic Acid Stains ..................................................................................................................................... TOTO and YOYO Dimeric Cyanine Dyes ............................................................................................................................................. Hoechst 33258, Hoechst 33342 and DAPI ......................................................................................................................................... Ethidium Bromide and Its Analogs ..................................................................................................................................................... Acridine Orange ................................................................................................................................................................................. 7-Aminoactinomycin D ...................................................................................................................................................................... Antibodies to Proliferation Markers and Cell-Cycle Control Proteins ......................................................................................................... Anti–cdc6 Peptide Antibody ............................................................................................................................................................... Anti–Human mRNA-Binding Protein HuR Antibody (Anti-HuR Antibody) .......................................................................................... Antibodies Against D Cyclins and Cyclin-Dependent Kinase Inhibitors .............................................................................................. Endostatin Protein and Angiostatin Protein for Angiogenesis Research .................................................................................................... Angiostatin ......................................................................................................................................................................................... Endostatin .......................................................................................................................................................................................... Data Table .................................................................................................................................................................................................. Product List ............................................................................................................................................................................................... 631 632 632 634 635 635 635 637 637 637 637 638 638 639 640 640 641 641 641 642 642 642 642 642 642 643 643 644 644 645 646 Section 15.5 — Assays for Apoptosis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 647 Apoptosis Assays Using Nucleic Acid Stains ............................................................................................................................................. Vybrant Apoptosis Assay Kit #4 ......................................................................................................................................................... Vybrant Apoptosis Assay Kits #5 and #7 ........................................................................................................................................... Comet (Single-Cell Gel Electrophoresis) Assay to Detect Damaged DNA ........................................................................................... Detecting DNA Strand Breaks with ChromaTide Nucleotides ..................................................................................................................... APO-BrdU TUNEL Assay Kit ...................................................................................................................................................................... 600 Chapter 15 — Assays for Cell Viability, Proliferation and Function 647 649 649 650 650 651 www.probes.com Apoptosis Assays Using Annexin V Conjugates ......................................................................................................................................... Annexin V Conjugates ........................................................................................................................................................................ Vybrant Apoptosis Assay Kit #1 ......................................................................................................................................................... Vybrant Apoptosis Assay Kit #2 ......................................................................................................................................................... Vybrant Apoptosis Assay Kit #3 ......................................................................................................................................................... Vybrant Apoptosis Assay Kit #6 ......................................................................................................................................................... Apoptosis Assays Based on Protease Activity ........................................................................................................................................... Caspases ............................................................................................................................................................................................ Caspase-3 Substrates and Assay Kits ................................................................................................................................................ Caspase-8 Substrates ........................................................................................................................................................................ Cathepsins and Calpains .................................................................................................................................................................... Apoptosis Assays Using Mitochondrial Stains ........................................................................................................................................... Apoptosis Assays Using Free Radical Probes ............................................................................................................................................ Apoptosis Assays Using Ion Indicators ..................................................................................................................................................... Apoptosis Assays Using Esterase Substrates ............................................................................................................................................ An Apoptosis Assay that Measures the ATP:ADP Ratio ............................................................................................................................. Data Table .................................................................................................................................................................................................. Product List ............................................................................................................................................................................................... 651 651 653 653 653 653 653 653 653 654 654 655 656 656 657 657 658 659 Section 15.6 — Probes for Cell Adhesion, Chemotaxis, Multidrug Resistance and Glutathione . . . . . 660 Cell Adhesion Assays ................................................................................................................................................................................. Cell Adhesion Assays Using Enzyme Substrates ................................................................................................................................ Vybrant Cell Adhesion Assay Kit ........................................................................................................................................................ Cell Adhesion Assays Using the CyQUANT Cell Proliferation Assay Kit .............................................................................................. Microsphere Adhesion Assays ........................................................................................................................................................... Fluorescent Fibrinogen ....................................................................................................................................................................... Fluorescent Gelatin and Type IV Collagen .......................................................................................................................................... Anti-Fibronectin Antibody .......................................................................................................................................................................... Chemotaxis Assays .................................................................................................................................................................................... Direct Detection of Chemotaxis Using Cell Counting .......................................................................................................................... Probes for Chemotaxis Receptors ...................................................................................................................................................... BODIPY FL Cytochalasin B: A Chemotaxis Inhibitor ........................................................................................................................... Multidrug Resistance Assays ..................................................................................................................................................................... MDR Assays Using Acetoxymethyl Esters ......................................................................................................................................... Vybrant Multidrug Resistance Assay Kit ............................................................................................................................................ Diagnostic Assay for Multidrug Resistance ........................................................................................................................................ MDR Assays Using Glutathione-Reactive Probes ............................................................................................................................... MDR Assays Using Mitochondrial Probes ......................................................................................................................................... MDR Assays Using Nucleic Acid Stains ............................................................................................................................................. BODIPY Derivatives of Verapamil, a Dihydropyridine and Vinblastine ................................................................................................ BODIPY Prazosin and BODIPY Forskolin ........................................................................................................................................... MDR Assays Using Ion Indicators ..................................................................................................................................................... Methotrexate Resistance and Gene Amplification .............................................................................................................................. Glutathione Determination ......................................................................................................................................................................... Glutathione Determination with Monochlorobimane .......................................................................................................................... Glutathione Determination with Visible Light–Excitable Thiol-Reactive Probes .................................................................................. PFB Aminofluorescein: A Specific Substrate for Glutathione S -Transferase ....................................................................................... Thiol Determination With o -Phthaldialdehyde and Naphthalene-2,3-dicarboxaldehyde ..................................................................... Data Table .................................................................................................................................................................................................. Product List ............................................................................................................................................................................................... 660 660 661 662 662 662 663 663 663 663 664 664 664 664 665 665 665 666 666 666 666 666 666 666 667 667 668 668 669 670 601 Hoechst 33342, tetramethylrhodamine ethyl ester and rhodamine 110, bis-L-aspartic acid amide Alexa Fluor 488 annexin V and propidium iodide LIVE/DEAD BacLight Bacterial Viability Kit 602 Chapter 15 — Assays for Cell Viability, Proliferation and Function www.probes.com
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