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Publication
Identification of the Mycobacterium ulcerans protein MUL_3720 as a
promising target for the development of a diagnostic test for Buruli
ulcer
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
ID 2980480
Author(s) Dreyer, Anita; Röltgen, Katharina; Dangy, Jean Pierre; Ruf, Marie Thérèse; Scherr, Nicole; Bolz,
Miriam; Tobias, Nicholas Jay; Moes, Charles; Vettiger, Andrea; Stinear, Timothy Paul; Pluschke, Gerd
Author(s) at UniBasel Ruf, Marie-Thérèse; Scherr, Nicole; Bolz, Miriam; Pluschke, Gerd;
Year 2015
Title Identification of the Mycobacterium ulcerans protein MUL_3720 as a promising target for the
development of a diagnostic test for Buruli ulcer
Journal PLoS neglected tropical diseases
Volume 9
Number 2
Pages / Article-Number e0003477
Buruli ulcer (BU) caused by Mycobacterium ulcerans is a devastating skin disease, occurring mainly in remote
West African communities with poor access to health care. Early case detection and subsequent antibiotic
treatment are essential to counteract the progression of the characteristic chronic ulcerative lesions. Since the
accuracy of clinical BU diagnosis is limited, laboratory reconfirmation is crucial. However, currently available
diagnostic techniques with sufficient sensitivity and specificity require infrastructure and resources only
accessible at a few reference centres in the African endemic countries. Hence, the development of a simple,
rapid, sensitive and specific point-of-care diagnostic tool is one of the major research priorities for BU. In this
study, we have identified a previously unknown M. ulcerans protein, MUL_3720, as a promising target for
antigen capture-based detection assays. We show that MUL_3720 is highly expressed by M. ulcerans and has
no orthologs in other prevalent pathogenic mycobacteria. We generated a panel of anti-MUL_3720 antibodies
and used them to confirm a cell wall location for MUL_3720. These antibodies could also specifically detect M.
ulcerans in infected human tissue samples as well as in lysates of infected mouse footpads. A bacterial 2hybrid screen suggested a potential role for MUL_3720 in cell wall biosynthesis pathways. Finally, we
demonstrate that a combination of MUL_3720 specific antibody reagents in a sandwich-ELISA format has
sufficient sensitivity to make them suitable for the development of antigen capture-based diagnostic tests for
BU.
Publisher Public Library of Science
ISSN/ISBN 1935-2727
edoc-URL http://edoc.unibas.ch/dok/A6348614
Full Text on edoc
Available
Digital Object Identifier DOI 10.1371/journal.pntd.0003477
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/25668636
ISI-Number WOS:000350992500027
Document type (ISI) Journal Article
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