Download STUDY ON THE CLINIC VALUE OF ABNORMAL GENE

STUDY ON THE CLINIC VALUE OF ABNORMAL GENE EXPRESSION ON
TRANSFORMING GROWTH FACTOR RECEPTOR Ⅱ IN PATHOLOGICAL SCAR
EPIDERMIS
CHEN Ming-rui, AN Gang, LIU Shun-li, WEI Feng- cai
Department of Burns and Plastic Surgery, the General Hospital of Jinan Military Region,
Jinan, Shandong 250032,China
Email: [email protected]
[Abstract ]
Objective: To investigate the mutation of transforming growth factor-β
receptorⅡ（TβRⅡ）in pathological scar epidermis and to discuss the clinical value.
Methods: Pathological scar epidermis of 20 burn or trauma patients from Qilu Hospital of
Shandong University and the general hospital of Jinan military region were collected from
2007 to 2009. 20 epidermal layer specimens were cut from the middle and edge of
pathological scar,separately. Also 20 normal skin specimens away from the lesion site of
more than 10 cm keep were collected as a body-control.And blood in 20 cases of pathological
scar patients was collected for self-control. Examples from normal skin of 8 cases with
healthy skin were collected for negative-control. Immunohistochemistry with biotinStreptavidin-peroxidase staining were used to detect the positive expression of TβRⅡin the
specimen (except blood specimens). PCR-SSCP and gene sequencing of TβRⅡgene
expression were compared.
Results: Immunohistochemistry showed that TβRⅡ express weakly in pathological scar
epidermis, which mainly located in the basal layer, cell,while the expression of TβRⅡseldom
or missing. TβRⅡ poly A fragments band of pathological scar epidermal specimens was
found swimming abnormalities and DNA fragment shortened by gene sequencing. No
abnormality was found in TβRⅡof normal skin and blood with pathological scar patients
and normal skin from patients with healthy skin.
Conclusion There is abnormal gene expression and mutation of TβRⅡin pathological scar
epidermis in the investigatecar. Epidermis back transplantation maybe risk scar recurring.
[Key words] Pathological scar; Transforming growth factor-β receptorⅡ（TβRⅡ; Genic
expression; Epidermis.