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STUDY ON THE CLINIC VALUE OF ABNORMAL GENE EXPRESSION ON TRANSFORMING GROWTH FACTOR RECEPTOR Ⅱ IN PATHOLOGICAL SCAR EPIDERMIS CHEN Ming-rui, AN Gang, LIU Shun-li, WEI Feng- cai Department of Burns and Plastic Surgery, the General Hospital of Jinan Military Region, Jinan, Shandong 250032,China Email: [email protected] [Abstract ] Objective: To investigate the mutation of transforming growth factor-β receptorⅡ(TβRⅡ)in pathological scar epidermis and to discuss the clinical value. Methods: Pathological scar epidermis of 20 burn or trauma patients from Qilu Hospital of Shandong University and the general hospital of Jinan military region were collected from 2007 to 2009. 20 epidermal layer specimens were cut from the middle and edge of pathological scar,separately. Also 20 normal skin specimens away from the lesion site of more than 10 cm keep were collected as a body-control.And blood in 20 cases of pathological scar patients was collected for self-control. Examples from normal skin of 8 cases with healthy skin were collected for negative-control. Immunohistochemistry with biotinStreptavidin-peroxidase staining were used to detect the positive expression of TβRⅡin the specimen (except blood specimens). PCR-SSCP and gene sequencing of TβRⅡgene expression were compared. Results: Immunohistochemistry showed that TβRⅡ express weakly in pathological scar epidermis, which mainly located in the basal layer, cell,while the expression of TβRⅡseldom or missing. TβRⅡ poly A fragments band of pathological scar epidermal specimens was found swimming abnormalities and DNA fragment shortened by gene sequencing. No abnormality was found in TβRⅡof normal skin and blood with pathological scar patients and normal skin from patients with healthy skin. Conclusion There is abnormal gene expression and mutation of TβRⅡin pathological scar epidermis in the investigatecar. Epidermis back transplantation maybe risk scar recurring. [Key words] Pathological scar; Transforming growth factor-β receptorⅡ(TβRⅡ; Genic expression; Epidermis.