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Mcl-1 is a common target of stem cell factor and interleukin-5 for apoptosis prevention activity via MEK/MAPK and PI-3K/Akt pathways by Huei-Mei Huang, Chang-Jen Huang, and Jeffrey Jong-Young Yen Blood Volume 96(5):1764-1771 September 1, 2000 ©2000 by American Society of Hematology IL-5 is unable to suppress apoptosis or induce Mcl-1 in TF-1 cells.(A) IL-5 suppressed apoptosis in JYTF-1, but not in TF-1 cells. Huei-Mei Huang et al. Blood 2000;96:1764-1771 ©2000 by American Society of Hematology Proliferation synergism of SCF correlates with Mcl-1 inducibility.(A) Requirement of SCF costimulation for optimal proliferation of TF-1 cell line. Huei-Mei Huang et al. Blood 2000;96:1764-1771 ©2000 by American Society of Hematology Differential phosphorylation of signaling components by hIL-5 in TF-1 and JYTF-1 cells.Both TF1 and JYTF-1 cells were cytokine-depleted for 24 hours (lanes 1 and 4) before stimulation with GM-CSF (lanes 2 and 5) and IL-5 (lanes 3 and 6) (as indicated above... Huei-Mei Huang et al. Blood 2000;96:1764-1771 ©2000 by American Society of Hematology Complementation effect of SCF costimulation on various signaling pathways.TF-1 and JYTF-1 cells were treated as described in Figure 2B, except that cytokine stimulation was for only 5 minutes, and cell lysates were analyzed for the activation of STAT5, MAPK... Huei-Mei Huang et al. Blood 2000;96:1764-1771 ©2000 by American Society of Hematology Effect of kinase inhibitors on the growth activity of IL-5 and SCF in JYTF-1 cells.(A) Differential growth properties to inhibitors in cells cultured in IL-5 and SCF. JYTF-1 cells were seeded into media containing IL-5 or SCF with or without PD98059 (100 μm... Huei-Mei Huang et al. Blood 2000;96:1764-1771 ©2000 by American Society of Hematology Inhibition of cytokine-induced activation of MAPK and Akt by kinase inhibitors.(A) Inhibition of phosphorylation of MAPK and Akt by kinase inhibitors. Huei-Mei Huang et al. Blood 2000;96:1764-1771 ©2000 by American Society of Hematology Down-regulation of Mcl-1 expression by kinase inhibitors.JYTF-1 cells were treated as described in Figure 6A and cell lysates were analyzed for the expression levels of Mcl-1, Bcl-2, and Bax, as described in Figure 1B. Huei-Mei Huang et al. Blood 2000;96:1764-1771 ©2000 by American Society of Hematology No suppression on the tyrosine phosphorylation of STAT5 by the kinase inhibitors.JYTF-1 cells were treated with PD98059 and LY294002 as described in Figure 6A. Huei-Mei Huang et al. Blood 2000;96:1764-1771 ©2000 by American Society of Hematology Schematic presentation of the hypothetical role of Mcl-1 in IL-5 and SCF antiapoptotic signaling pathways.LY indicates LY294002 and is the PI-3K inhibitor. Huei-Mei Huang et al. Blood 2000;96:1764-1771 ©2000 by American Society of Hematology
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