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Anticancer Properties of Vaticanol B from Stem Bark of
Dipterocarpaceae Toward Human Cancer Cell Lines.
Retno Arianingrum*, Sri Atun, and Nurfina Aznam
Department of Chemistry Education, Faculty of Mathematic and Natural Science, Yogyakarta State
University, Karangmalang, Yogyakarta 55281, Indonesia
*E-mail: [email protected]., Tel: +62-0274-586168 ext 217 or +62-0817260172
This study investigates the cytotoxic activity, antiproliferative effect, and how the antiproliferative
mechanism of vaticanol B from stem bark of Dipterocarpaceae towards HeLa, Raji, and Myeloma
human cancer cell lines. The toxicity and the effect of vaticanol B on cell proliferation inhibition
was measured by MTT assay. Apoptosis detection was done by double staining using ethidium
bromide/acrydine orange and observed on fluorescence microscope. The expression of p53 was
determined by immunohistochemical analysis. The data obtained indicate that vaticanol B inhibited
the proliferation of HeLa, Raji and Myeloma human cancer cell lines with IC50 values of 92.811
g/ml, 67.066 g/ml, and 95.499 g/ml respectively. The compound had no cytotoxic effect
towards normal cell line, Vero. The analysis of cell doubling time indicated that vaticanol B had
effect on cell cycle progression by defer cell doubling. This compound was able to induce apoptosis
by influence the expression of p53.